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首页> 美国卫生研究院文献>Genes >The Chloroplast Genome Sequence of Scutellaria baicalensis Provides Insight into Intraspecific and Interspecific Chloroplast Genome Diversity in Scutellaria
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The Chloroplast Genome Sequence of Scutellaria baicalensis Provides Insight into Intraspecific and Interspecific Chloroplast Genome Diversity in Scutellaria

机译：黄ical叶绿体基因组序列为黄cut种内和种间叶绿体基因组多样性提供了见识

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摘要

Scutellaria baicalensis Georgi (Lamiaceae) is the source of the well-known traditional Chinese medicine “HuangQin” (Radix Scutellariae). Natural sources of S. baicalensis are rapidly declining due to high market demand and overexploitation. Moreover, the commercial products of Radix Scutellariae have often been found to contain adulterants in recent years, which may give rise to issues regarding drug efficacy and safety. In this study, we developed valuable chloroplast molecular resources by comparing intraspecific and interspecific chloroplast genome. The S. baicalensis chloroplast genome is a circular molecule consisting of two single-copy regions separated by a pair of inverted repeats. Comparative analyses of three Scutellaria chloroplast genomes revealed six variable regions (trnH-psbA, trnK-rps16, petN-psbM, trnT-trnL, petA-psbJ, and ycf1) that could be used as DNA barcodes. There were 25 single nucleotide polymorphisms(SNPs) and 29 indels between the two S. baicalensis genotypes. All of the indels occurred within non-coding regions. Phylogenetic analysis suggested that Scutellarioideae is a sister taxon to Lamioideae. These resources could be used to explore the variation present in Scutellaria populations and for further evolutionary, phylogenetic, barcoding and genetic engineering studies, in addition to effective exploration and conservation of S. baicalensis.
机译：黄cut药草（黄ami科）是著名的中药“黄秦”（黄x）的来源。由于市场需求旺盛和过度开发，黄ical的天然来源正在迅速减少。此外，近年来经常发现黄Rad的商业产品含有掺假物，这可能引起有关药物功效和安全性的问题。在这项研究中，我们通过比较种内和种间叶绿体基因组开发了有价值的叶绿体分子资源。黄ical叶绿体基因组是由两个单拷贝区域组成的环状分子，该区域由一对反向重复序列隔开。对三个黄cut叶绿体基因组的比较分析显示了六个可变区（trnH-psbA，trnK-rps16，petN-psbM，trnT-trnL，petA-psbJ和ycf1），可用作DNA条码。两种S. baicalensis基因型之间有25个单核苷酸多态性（SNPs）和29个插入缺失。所有插入缺失均发生在非编码区内。系统发育分析表明，黄cut科是唇形科的姊妹分类群。这些资源可用于探索黄cut种群中的变异，以及有效地探索和保护黄ical菌，进而用于进一步的进化，系统发育，条形码和基因工程研究。

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