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首页> 美国卫生研究院文献>Enzyme Research >Optimal Conditions for Continuous Immobilization of Pseudozyma hubeiensis (Strain HB85A) Lipase by Adsorption in a Packed-Bed Reactor by Response Surface Methodology
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Optimal Conditions for Continuous Immobilization of Pseudozyma hubeiensis (Strain HB85A) Lipase by Adsorption in a Packed-Bed Reactor by Response Surface Methodology

机译：响应面方法在填充床反应器中连续吸附固定湖北假单胞菌（HB85A菌株）脂肪酶的最佳条件

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This study aimed to develop an optimal continuous process for lipase immobilization in a bed reactor in order to investigate the possibility of large-scale production. An extracellular lipase of Pseudozyma hubeiensis (strain HB85A) was immobilized by adsorption onto a polystyrene-divinylbenzene support. Furthermore, response surface methodology (RSM) was employed to optimize enzyme immobilization and evaluate the optimum temperature and pH for free and immobilized enzyme. The optimal immobilization conditions observed were 150 min incubation time, pH 4.76, and an enzyme/support ratio of 1282 U/g support. Optimal activity temperature for free and immobilized enzyme was found to be 68°C and 52°C, respectively. Optimal activity pH for free and immobilized lipase was pH 4.6 and 6.0, respectively. Lipase immobilization resulted in improved enzyme stability in the presence of nonionic detergents, at high temperatures, at acidic and neutral pH, and at high concentrations of organic solvents such as 2-propanol, methanol, and acetone.
机译：这项研究旨在开发一种最佳的连续工艺，用于固定床反应器中的脂肪酶，以研究大规模生产的可能性。通过吸附到聚苯乙烯-二乙烯基苯载体上来固定湖北假单胞菌的细胞外脂肪酶（菌株HB85A）。此外，采用响应表面方法（RSM）来优化酶的固定化，并评估游离和固定化酶的最佳温度和pH。观察到的最佳固定条件是孵育时间为150分钟，pH为4.76，酶/载体比为1282U / g。发现游离和固定化酶的最佳活性温度分别为68℃和52℃。游离和固定化脂肪酶的最佳活性pH分别为4.6和6.0。在非离子型去污剂的存在下，在高温，酸性和中性pH以及高浓度的有机溶剂（例如2-丙醇，甲醇和丙酮）下，脂肪酶的固定化可改善酶的稳定性。

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