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首页> 美国卫生研究院文献>Environmental Health Perspectives >The fate of trenbolone acetate and melengestrol acetate after application as growth promoters in cattle: environmental studies.
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The fate of trenbolone acetate and melengestrol acetate after application as growth promoters in cattle: environmental studies.

机译：醋酸群勃龙和醋酸美仑孕酮作为牛生长促进剂后的命运：环境研究。

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The steroids trenbolone acetate (TbA) and melengestrol acetate (MGA) are licensed as growth promoters for farm animals in several meat-exporting countries. Although many studies have explored their safety for both animals and consumers, little is known about their fate after excretion by the animal. Our study aimed to determine the residues and degradation of trenbolone and MGA in solid dung, liquid manure, and soil. In animal experiments lasting 8 weeks, cattle were treated with TbA and MGA. Solid dung and, in case of trenbolone, liquid manure were collected and spread on maize fields after 4.5 and 5.5 months of storage, respectively. Determination of the hormone residues in all samples included extraction, clean-up (solid-phase extraction), separation of metabolites and interfering substances by HPLC (RP-18), and quantification by sensitive enzyme immunoassay. Procedures were validated by mass spectrometry (MS) methods. During storage of liquid manure the level of trenbolone decreased from 1,700 to 1,100 pg/g (17alpha-isomer), corresponding to a half-life of 267 days. Before storage, the concentrations in the dung hill ranged from 5 to 75 ng/g TbOH and from 0.3 to 8 ng/g MGA. After storage, levels up to 10 ng/g trenbolone, and 6 ng/g MGA were detected. In the soil samples trenbolone was traceable up to 8 weeks after fertilization, and MGA was detected even until the end of the cultivation period. The results show that these substances should be investigated further concerning their potential endocrine-disrupting activity in agricultural ecosystems.
机译：类固醇乙酸群勃龙（TbA）和乙酸美仑孕酮（MGA）被许可在几个肉类出口国用作农场动物的生长促进剂。尽管许多研究都探讨了它们对动物和消费者的安全性，但对它们被动物排泄后的命运知之甚少。我们的研究旨在确定群勃龙和MGA在固体粪便，液体肥料和土壤中的残留和降解。在持续8周的动物实验中，牛用TbA和MGA处理。分别储存4.5个月和5.5个月后，收集固体粪便和群勃龙（如果使用群勃龙）粪便，并将其撒在玉米田中。所有样品中激素残留的测定包括提取，纯化（固相提取），通过HPLC（RP-18）分离代谢产物和干扰物质以及通过灵敏的酶免疫法进行定量。通过质谱（MS）方法验证了程序。在储存液态肥料的过程中，群勃龙的含量从1,700 pg / g（17α-异构体）降低至1,100 pg / g，对应半衰期为267天。储存前，粪堆中的TbOH浓度为5至75 ng / g MGA，0.3至8 ng / g MGA。储存后，检测到的浓度高达10 ng / g群勃龙和6 ng / g MGA。在土壤样品中，群勃龙在施肥后长达8周都是可追溯的，甚至在培养期结束之前都检测到了MGA。结果表明，应进一步研究这些物质在农业生态系统中的潜在破坏内分泌的活性。

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