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Differential early viral gene expression in two stages of human papillomavirus type 16 DNA-induced malignant transformation.

机译：在人类乳头瘤病毒16型DNA诱导的恶性转化的两个阶段中差异性早期病毒基因表达。

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Human papillomavirus (HPV) type 16 DNA induces progressive transformation in NIH 3T3 cells. Two types of cell lines, PM3T3G0 and PM3T3Fo, were isolated by G418 or focus selection, respectively, after transfection of cells by a recombinant HPV 16 DNA carrying the neo gene. These cell lines exhibited distinct phenotypes compared with controls. Saturation densities of PM3T3G0 and PM3T3Fo lines were two- to three- and five- to sevenfold greater than that of control NIH 3T3 cells, respectively. Neither cell type required high serum for growth, in contrast to NIH 3T3 cells. PM3T3G0 lines were premalignant, whereas PM3T3Fo lines manifested tumorigenicity within 2 weeks. Subpopulations of three PM3T3G0 lines underwent progressive transformation as reflected by focus formation. Analysis of HPV 16-specific mRNA species demonstrated that high levels of early and late gene expression were detected in premalignant PM3T3G0 lines, whereas relatively low quantities of selected gene messages were expressed in malignant transformants. Thus, high levels of viral gene expression are not crucial for malignant transformation.

机译：人类乳头瘤病毒（HPV）16型DNA诱导NIH 3T3细胞进行性转化。在通过携带neo基因的重组HPV 16 DNA转染细胞后，分别通过G418或焦点选择分离了两种类型的细胞系PM3T3G0和PM3T3Fo。与对照相比，这些细胞系表现出不同的表型。 PM3T3G0和PM3T3Fo系的饱和密度分别比对照NIH 3T3细胞高2到3倍和5到7倍。与NIH 3T3细胞相比，这两种细胞都不需要高血清来生长。 PM3T3G0系是恶性的，而PM3T3Fo系在2周内表现出致癌性。正如焦点形成所反映的那样，三个PM3T3G0系的亚群经历了逐步转化。对HPV 16特异性mRNA种类的分析表明，在恶变前的PM3T3G0品系中检测到高水平的早期和晚期基因表达，而在恶性转化株中表达的基因信息相对较少。因此，高水平的病毒基因表达对于恶性转化不是至关重要的。

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期刊名称 Molecular and Cellular Biology
作者
S Yasumoto; J Doniger; J A DiPaolo;
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年(卷),期 1987(7),6
年度 1987
页码 2165–2172
总页数 8
原文格式 PDF
正文语种
中图分类分子生物学;细胞生物学;
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1. Differential early viral gene expression in two stages of human papillomavirus type 16 DNA-induced malignant transformation. [J] . S Yasumoto, J Doniger, J A DiPaolo Molecular and Cellular Biology . 1987,第6期

机译：人乳头瘤病毒16型DNA诱导恶性转化的差异早期病毒基因表达。
2. Differential early viral gene expression in two stages of human papillomavirus type 16 DNA-induced malignant transformation. [J] . S Yasumoto, J Doniger, J A DiPaolo Molecular and Cellular Biology . 1987,第6期

机译：人乳头瘤病毒16型DNA诱导恶性转化的差异早期病毒基因表达。
3. Differential early viral gene expression in two stages of human papillomavirus type 16 DNA-induced malignant transformation. [J] . S Yasumoto, J Doniger, J A DiPaolo Molecular and Cellular Biology . 1987,第6期

机译：人乳头瘤病毒16型DNA诱导恶性转化的差异早期病毒基因表达。
4. DIFFERENTIAL ANTIBODY RESPONSE AGAINST CONFORMATIONAL AND LINEAR EPITOPES OF THE L1 PROTEIN FROM HUMAN PAPILLOMAVIRUS TYPES 16/18 IS GENERATED IN VACCINATED WOMAN OR WITH DIFFERENT EXPOSURES TO THE VIRUS. [C] . Gutierrez-Xicotencatl L, Pedroza-Saavedra A, Rodriguez-Ocampo AN, Vaccine technology VI . 2016

机译：针对人乳头瘤病毒类型16/18的L1蛋白质的构象和线性表位产生的不同抗体反应是在疫苗接种的妇女中产生的，或在与病毒的不同接触中产生的。
5. Modulation of human papillomavirus type 16 early gene expression by transforming growth factor-beta. [D] . Baldwin, Amy. 2003

机译：通过转化生长因子-β调节人乳头瘤病毒16型早期基因表达。
6. Human papillomavirus type 16 DNA-induced malignant transformation of NIH 3T3 cells. [O] . S Yasumoto, A L Burkhardt, J Doniger, 1986

机译：人乳头瘤病毒16型DNA诱导的NIH 3T3细胞恶性转化。
7. Differential early viral gene expression in two stages of human papillomavirus type 16 DNA-induced malignant transformation. [O] . Yasumoto, S, Doniger, J, DiPaolo, J A 1987

机译：在人类乳头瘤病毒16型DNA诱导的恶性转化的两个阶段中，差异性早期病毒基因表达。

Differential early viral gene expression in two stages of human papillomavirus type 16 DNA-induced malignant transformation.

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