Differential early viral gene expression in two stages of human papillomavirus type 16 DNA-induced malignant transformation.

S Yasumoto; J Doniger; J A DiPaolo

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Differential early viral gene expression in two stages of human papillomavirus type 16 DNA-induced malignant transformation.

机译：人乳头瘤病毒16型DNA诱导恶性转化的差异早期病毒基因表达。

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Human papillomavirus (HPV) type 16 DNA induces progressive transformation in NIH 3T3 cells. Two types of cell lines, PM3T3G0 and PM3T3Fo, were isolated by G418 or focus selection, respectively, after transfection of cells by a recombinant HPV 16 DNA carrying the neo gene. These cell lines exhibited distinct phenotypes compared with controls. Saturation densities of PM3T3G0 and PM3T3Fo lines were two- to three- and five- to sevenfold greater than that of control NIH 3T3 cells, respectively. Neither cell type required high serum for growth, in contrast to NIH 3T3 cells. PM3T3G0 lines were premalignant, whereas PM3T3Fo lines manifested tumorigenicity within 2 weeks. Subpopulations of three PM3T3G0 lines underwent progressive transformation as reflected by focus formation. Analysis of HPV 16-specific mRNA species demonstrated that high levels of early and late gene expression were detected in premalignant PM3T3G0 lines, whereas relatively low quantities of selected gene messages were expressed in malignant transformants. Thus, high levels of viral gene expression are not crucial for malignant transformation.

机译：人乳头瘤病毒（HPV）16 DNA在NIH 3T3细胞中诱导逐渐转化。通过G418或聚焦选择在通过携带NEO基因的重组HPV 16 DNA转染细胞后，分别分离两种类型的细胞系，PM3T3G0和PM3T3FO。与对照相比，这些细胞系表现出明显的表型。 PM3T3G0和PM3T3FO线的饱和密度分别分别比对照NIH 3T3细胞的2至三到七倍。与NIH 3T3细胞相比，既不需要高血清用于生长。 PM3T3G0系列是前美的，而PM3T3FO系列在2周内表现出致瘤性。三种PM3T3G0系的亚步进焦点形成反映的逐方转化。 HPV 16特异性mRNA物种的分析证明，在预急性的PM3T3G0系中检测到高水平的早期和晚期基因表达，而在恶性转化体中表达相对较少量的选择基因。因此，高水平的病毒基因表达对于恶性转化不至关重要。

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《Molecular and Cellular Biology》 |1987年第6期|共8页
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S Yasumoto; J Doniger; J A DiPaolo;
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1. Differential early viral gene expression in two stages of human papillomavirus type 16 DNA-induced malignant transformation. [J] . S Yasumoto, J Doniger, J A DiPaolo Molecular and Cellular Biology . 1987,第6期

机译：人乳头瘤病毒16型DNA诱导恶性转化的差异早期病毒基因表达。
2. Differential early viral gene expression in two stages of human papillomavirus type 16 DNA-induced malignant transformation. [J] . S Yasumoto, J Doniger, J A DiPaolo Molecular and Cellular Biology . 1987,第6期

机译：人乳头瘤病毒16型DNA诱导恶性转化的差异早期病毒基因表达。
3. Effects of 12-O-tetradecanoylphorbol-13-acetate on human papillomavirus type 16-positive keratinocytes at different stages of transformation. [J] . Sears WL, Goto Mandeville R, Mirapuri M, Molecular Carcinogenesis . 1995,第3期

机译：12-O-十四烷酰佛波醇-13-乙酸盐在不同转化阶段对人乳头瘤病毒16型阳性角质形成细胞的影响。
4. DIFFERENTIAL ANTIBODY RESPONSE AGAINST CONFORMATIONAL AND LINEAR EPITOPES OF THE L1 PROTEIN FROM HUMAN PAPILLOMAVIRUS TYPES 16/18 IS GENERATED IN VACCINATED WOMAN OR WITH DIFFERENT EXPOSURES TO THE VIRUS. [C] . Gutierrez-Xicotencatl L, Pedroza-Saavedra A, Rodriguez-Ocampo AN, Vaccine technology VI . 2016

机译：针对人乳头瘤病毒类型16/18的L1蛋白质的构象和线性表位产生的不同抗体反应是在疫苗接种的妇女中产生的，或在与病毒的不同接触中产生的。
5. Modulation of human papillomavirus type 16 early gene expression by transforming growth factor-beta. [D] . Baldwin, Amy. 2003

机译：通过转化生长因子-β调节人乳头瘤病毒16型早期基因表达。
6. Differential early viral gene expression in two stages of human papillomavirus type 16 DNA-induced malignant transformation. [O] . S Yasumoto, J Doniger, J A DiPaolo 1987

机译：在人类乳头瘤病毒16型DNA诱导的恶性转化的两个阶段中差异性早期病毒基因表达。
7. Differential early viral gene expression in two stages of human papillomavirus type 16 DNA-induced malignant transformation. [O] . Yasumoto, S, Doniger, J, DiPaolo, J A 1987

机译：在人类乳头瘤病毒16型DNA诱导的恶性转化的两个阶段中，差异性早期病毒基因表达。

Differential early viral gene expression in two stages of human papillomavirus type 16 DNA-induced malignant transformation.

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