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UPLC-MS/MS Method for the Determination of 14 Compounds in Rat Plasma and Its Application in a Pharmacokinetic Study of Orally Administered Xiaoyao Powder

机译：UPLC-MS / MS法测定大鼠血浆中14种化合物的含量及其在口服逍遥散的药代动力学研究中的应用

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摘要

Xiaoyao Powder (XYP), a common Chinese medicine, comprises eight traditional Chinese herbs and has been widely used clinically to treat liver damage and mental disorders. An ultra-performance liquid chromatography–tandem mass spectrometry method was developed to investigate the pharmacokinetics of 14 compounds (albiflorin, paeoniflorin, ferulic acid, senkyunolide I, quercetin, isoliquiritigenin, atractylenolide III, ligustilide, atractylenolide II, liquiritin, liquiritigenin, saikosaponin c, glycyrrhizic acid, and saikosaponin a) in XYP. Naringenin was used as the internal standard. The compounds were separated using an ACQUITY UPLCTM BEH C18 column (1.7 μm, 50 × 2.1 mm) with a mobile phase consisting of acetonitrile and 0.1% formic acid in water at a flow rate of 0.3 mL/min. Detection was performed on a triple-quadrupole tandem mass spectrometer using multiple reaction monitoring and an electrospray ionization source in both positive and negative ionization modes. All calibration curves exhibited good linearity (r2 > 0.9974) over the measured ranges. The intra- and inter-day precisions were within 12%, and the accuracy ranged from 89.93% to 106.64%. Extraction recovery and matrix effect results were satisfactory. The method was successfully applied in a pharmacokinetic study of the 14 compounds in rat plasma after the oral administration of XYP.
机译：逍遥散（XYP）是一种常见的中药，包含八种中草药，已在临床上广泛用于治疗肝损伤和精神障碍。开发了一种超高效液相色谱-串联质谱法研究14种化合物的药代动力学（白花素，flor药花青素，阿魏酸，千古内酯I，槲皮素，异黄体生成素，白术烯内酯III，li古丁内酯，白术烯基内酯II，脂白蛋白，皂苷，皂苷XYP中的甘草酸和皂苷a）。柚皮素被用作内标。使用ACQUITY UPLC TM BEH C18色谱柱（1.7μm，50×2.1 mm）分离混合物，流动相由乙腈和0.1％甲酸的水溶液组成，流速为0.3 mL /分钟在三重四极杆串联质谱仪上使用正负电离模式的多重反应监测和电喷雾电离源进行检测。所有校准曲线在测量范围内均表现出良好的线性（r 2

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