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首页> 美国卫生研究院文献>Molecules >Development and Validation of a HPLC-MS/MS Method for Simultaneous Determination of Twelve Bioactive Compounds in Epimedium: Application to a Pharmacokinetic Study in Rats
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Development and Validation of a HPLC-MS/MS Method for Simultaneous Determination of Twelve Bioactive Compounds in Epimedium: Application to a Pharmacokinetic Study in Rats

机译：同时测定淫羊med中十二种生物活性化合物的HPLC-MS / MS方法的建立和验证：在大鼠药代动力学研究中的应用

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A rapid and reliable HPLC-MS/MS method has been developed and validated for the simultaneous quantification of twelve bioactive compounds (baohuoside II, baohuoside I, sagittatoside A, sagittatoside B, magnoflorine, epimedin A, epimedin B, epimedin C, chlorogenic acid, neochlorogenic acid, cryptochlorogenic acid and icariin) in rat plasma. The collected plasma samples were prepared by protein precipitate with acetonitrile. The twelve compounds were separated on a CORTECS®C18 column (4.6 mm × 150 mm, 2.7 μm) with a gradient mobile phase system of 0.1% (v/v) formic acid and acetonitrile at a flow rate of 0.3 mL/min. All of the analytes were quantitated using electrospray ionization (ESI) in negative ion mode with selected reaction monitoring (SRM). The intra- and inter-day accuracy ranged from −5.6% to 13.0%, and the precisions of the analytes were less than 10.9%. The mean recoveries of the analytes were in the range of 60.66% to 99.77% and the matrix effect ranged from 93.08% to 119.84%. Stability studies proved that the analytes were stable under the tested conditions, with a relative standard deviation (RSD) lower than 11.7%. The developed method was successfully applied to evaluating the pharmacokinetic study of twelve bioactive compounds after oral administration of Epimedium extract in rat.
机译：已开发出一种快速可靠的HPLC-MS / MS方法，并已用于同时定量十二种生物活性化合物的验证（保side苷II，保hu苷I，箭叶苷A，箭叶苷B，厚朴素，淫羊med苷A，淫羊in苷B，淫羊in苷C，绿原酸，大鼠血浆中的新绿原酸，隐绿原酸和二十碳素）。通过用乙腈沉淀蛋白质来制备收集的血浆样品。十二种化合物在CORTECS ® C18色谱柱（4.6 mm×150 mm，2.7μm）上以0.1％（v / v）甲酸和乙腈的梯度流动相系统进行分离0.3 mL / min。使用选定的反应监测（SRM），以负离子模式使用电喷雾电离（ESI）对所有分析物进行定量。日内和日间准确性在-5.6％到13.0％之间，并且分析物的准确性小于10.9％。分析物的平均回收率在60.66％至99.77％的范围内，基质效应在93.08％至119.84％的范围内。稳定性研究证明，分析物在测试条件下稳定，相对标准偏差（RSD）低于11.7％。所开发的方法成功地用于评价淫羊Epi提取物在大鼠内口服后对十二种生物活性化合物的药代动力学研究。

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