首页> 美国卫生研究院文献>Nucleic Acids Research >A new type of plasmid from a wild isolate of Dictyostelium species: the existence of closely situated long inverted repeats.
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A new type of plasmid from a wild isolate of Dictyostelium species: the existence of closely situated long inverted repeats.

机译:一种来自野生的盘基网柄菌属物种的新型质粒:存在紧密排列的长反向重复序列。

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摘要

A circular plasmid having high copy number was found in a wild isolate of Dictyostelium species. Gel electrophoresis, electron microscopy and Southern blot hybridization revealed that the plasmid, named pDG1, is 4.5 Kb (1.5 micron) in size with closely situated long inverted repeats. The plasmid seems to be located in the nuclei. It was not a derivative of ribosomal DNA. The possible correlation of the plasmid with the putative intermediate DNA of retrotransposon DIRS-1 found in Dictyostelium discoideum is discussed.
机译:在双歧杆菌属物种的野生分离物中发现了具有高拷贝数的环状质粒。凝胶电泳,电子显微镜和Southern印迹杂交表明,名为pDG1的质粒大小为4.5 Kb(1.5微米),并有很长的反向重复序列。质粒似乎位于细胞核中。它不是核糖体DNA的衍生物。讨论了该质粒与在盘基网柄菌中发现的逆转录转座子DIRS-1的假定中间DNA的可能相关性。

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