• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Nucleic Acids Research >Faithful expression of a heterologous gene carried on an artificial macronuclear chromosome in Euplotes crassus.
【2h】

Faithful expression of a heterologous gene carried on an artificial macronuclear chromosome in Euplotes crassus.

机译：真核表达真实存在于人工大核染色体上的异源基因。

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Macronuclear chromosomes of hypotrichous ciliates are gene-sized molecules carrying the coding sequence flanked by short non-translated regions and bounded by telomeres. We have constructed artificial chromosomes for investigation of transcription in the macronucleus of Euplotes crassus. The neo gene was put under the control of the 5"-non-translated region of the TBP gene of E.crassus. These molecules were introduced into the cell with the help of liposomes. The cells were transformed and survived high concentrations of geneticin. The artificial chromosomes were kept in the macro-nucleus for at least 50 days at a copy number of about 200 per macronucleus. Expression of the gene was shown by reverse transcription of the neo messenger. The transcription start was mapped and found to coincide with that found on the natural macronuclear chromosome encoding TBP in E.crassus.
机译：纤毛纤毛虫的大核染色体是基因大小的分子，带有编码序列，其侧翼为短的非翻译区，端粒为界。我们已经构建了人工染色体，用于研究真蟹真核大核中的转录。将neo基因置于E.crassus的TBP基因的5“-非翻译区的控制之下。这些分子借助脂质体被引入细胞。细胞被转化并在高浓度的遗传霉素中存活。人工染色体在大核中至少保留了50天，每个大核的拷贝数约为200，通过新信使的反转录显示基因的表达，并绘制了转录起点，发现与之相符。被发现在大肠埃希氏菌中编码TBP的天然大核染色体上。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号