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Effect of Glyphosate on Intact Bean Plants (Phaseolus vulgaris L.) and Isolated Cells

机译:草甘膦对完整豆类植物(菜豆)和分离细胞的影响

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摘要

Whole bean (var. “Eastern Butterwax”) plants and isolated cells were used to investigate possible mechanisms of action of glyphosate [N-(phosphonomethyl)glycine]. Results showed that glyphosate was quickly absorbed by the whole plant but not by individual cells and that it caused a rapid reduction in leaf dry matter accumulation, leaf expansion, leaf angle, and stomatal aperture without affecting the water status of the plant. Glyphosate also caused a rapid reduction in cellular uptake of 86Rb and 32P which preceded its detrimental effects on photosynthesis, RNA and protein synthesis, and respiration of isolated cells. This reduction in ion absorption was not due to a loss of membrane integrity, decrease in energy supply or chelation of ions. It was concluded that glyphosate was directly inhibiting the ion absorption process of bean leaf cells.
机译:使用全豆(变种“ Eastern Butterwax”)植物和分离的细胞研究草甘膦[N-(膦酰基甲基)甘氨酸]的可能作用机理。结果表明,草甘膦可被整个植物快速吸收,但不被单个细胞吸收,草甘膦可导致叶片干物质积累,叶片膨胀,叶片角度和气孔孔径迅速减少,而不会影响植物的水分状况。草甘膦还导致细胞对 86 Rb和 32 P的吸收迅速降低,这先于其对光合作用,RNA和蛋白质合成以及分离细胞呼吸的有害作用。离子吸收的降低不是由于膜完整性的损失,能量供应的减少或离子的螯合。结论是草甘膦直接抑制了豆叶细胞的离子吸收过程。

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