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首页> 外文期刊>Biotechnologie, Agronomie Societe et Environnement >Use of induced mutations in embryogenesis study in bean Phaseolus vulgaris L. and two model plants, Arabidopsis thaliana (L.) Heynh. and Zea mays L.Original Title (non-English) Utilisation des mutations induites pour l'etude de l'embryogenese chez le haricot Phaseolus vulgaris L. et deux plantes modeles, Arabidopsis thaliana (L.) Heynh. et Zea mays L. [French]
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Use of induced mutations in embryogenesis study in bean Phaseolus vulgaris L. and two model plants, Arabidopsis thaliana (L.) Heynh. and Zea mays L.Original Title (non-English) Utilisation des mutations induites pour l'etude de l'embryogenese chez le haricot Phaseolus vulgaris L. et deux plantes modeles, Arabidopsis thaliana (L.) Heynh. et Zea mays L. [French]

机译：在菜豆菜豆和两种模式植物拟南芥Heynh的胚发生研究中诱导突变的使用。最初的标题（非英语）用于诱导菜豆菜豆和两种模式植物拟南芥Heynh胚发生的诱变。和Zea maysL。[法语]

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Use of induced mutations in embryogenesis study in bean Phaseolus vulgaris L. and two model plants, Arabidopsis thaliana (L.) Heynh. and Zea mays L. Breeding of common bean, Phaseolus vulgaris L., through interspecific hybridizations with the species Phaseolus coccineus L. and Phaseolus polyanthus Greenm. as female parents leads to the abortion of immature embryos. Identification of genes required for embryo development could partly explain the abortion of hybrid embryos; induced mutations could thus be an alternative to identify key genes involved in Phaseolus embryogenesis. This paper is a review which shows a few examples of the use of induced mutations in the identification of essential genes for embryogenesis in two model plants, Arabidopsis thaliana (L.) Heyhn. for dicots and Zea mays L. for monocots. In these two species, embryo development mutants have been isolated using insertional mutagenesis and chemical mutagenesis with Ethyl Methane Sulfonate (EMS). Arabidopsis embryo mutants are affected in apical-basal axis polarity, radial pattern and in post-embryonic stages. Some Arabidopsis embryo mutants are defected in auxin signalisation. In maize, defective kernel (dek) mutants are affected in the embryo and the endosperm, while in embryo specific (emb) mutants, only the embryo is affected. In common bean, plants deficient in seed development were isolated using EMS mutagenesis. Embryos inside the seeds fail to growth at different stages of development and show abnormalities mainly in the suspensor and the cotyledons.
机译：在菜豆菜豆和两种模式植物拟南芥Heynh的胚发生研究中诱导突变的使用。通过与豆科菜豆和菜豆绿豆的种间杂交育种普通豆菜豆。因为女性父母导致未成熟胚胎的流产。鉴定胚胎发育所需的基因可以部分解释杂交胚胎的流产。因此，诱导的突变可能是鉴定参与菜豆胚发生的关键基因的替代方法。本文是一篇综述，它显示了在两个模式植物拟南芥（L.）Heyhn的胚发生必不可少的基因鉴定中使用诱导突变的一些实例。用于双子叶植物，Zea mays L.用于单子叶植物。在这两个物种中，已通过插入诱变和甲烷磺酸乙酯（EMS）的化学诱变分离了胚胎发育突变体。拟南芥胚胎突变体在根尖基轴极性，放射状和胚后阶段受到影响。一些拟南芥胚胎突变体在生长素信号转导中存在缺陷。在玉米中，有缺陷的仁（dek）突变体在胚和胚乳中受到影响，而在胚胎特异性（emb）突变体中，仅胚受到影响。在普通豆中，使用EMS诱变方法分离出种子发育欠佳的植物。种子内的胚胎在发育的不同阶段无法生长，并且主要在悬液和子叶中表现出异常。

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