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Specific Combinations of Zein Genes and Genetic Backgrounds Influence the Transcription of the Heavy-Chain Zein Genes in Maize opaque-2 Endosperms

机译：玉米醇溶蛋白基因和遗传背景的特定组合影响玉米不透明2胚乳中重链玉米醇溶蛋白基因的转录。

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摘要

The transcript levels of heavy-chain zein genes (zH1 and zH2) and the occurrence of the zH polypeptides in different opaque-2 (o2) lines were investigated by RNA-blot analyses and by sodium dodecylsulfate-polyacrylamide gel electrophoresis or two-dimensional gel electrophoresis protein fractionations. Four mutant alleles o2R, o2T, o2It, and o2-676 introgressed into different genetic backgrounds (GBs) were considered. The mono-dimensional gel electrophoresis zein pattern can be either conserved or different among the various GBs carrying the same o2 allele. Likewise, in the identical GB carrying different o2 alleles, the zein pattern can be either conserved or differentially affected by the different mutant allele. Zein protein analysis of reciprocal crosses between lines with different o2 alleles or the same o2 showed in some case a more than additive zH pattern in respect to the o2 parent lines. Electrophoretic mobility shift assay approaches, with O2-binding oligonucleotide and endosperm extracts from the above o2 lines, failed to reveal o2-specific retarded band in any of the o2 extracts. The results suggest that the promoter of some zH1 and zH2 contains motif(s) that can respond to factors other than O2.

机译：通过RNA印迹分析和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳或二维凝胶研究了重链玉米蛋白基因（zH1和zH2）的转录水平以及不同不透明2（o2）系中zH多肽的存在电泳蛋白质分离。考虑到渗入不同遗传背景（GB）的四个突变等位基因o2R，o2T，o2It和o2-676。一维凝胶电泳玉米醇溶蛋白模式可以是保守的，也可以在携带相同o2等位基因的各种GB之间不同。同样，在带有不同o2等位基因的相同GB中，玉米醇溶蛋白模式可以被保守或受不同突变等位基因的影响。对具有不同o2等位基因或相同o2的品系之间的反向杂交的玉米蛋白分析表明，在某些情况下，相对于o2亲本品系而言，zH模式具有更多的累加性。电泳迁移率变动分析方法（使用O2结合寡核苷酸和上述o2系的胚乳提取物）无法在任何o2提取物中揭示o2特异性阻滞带。结果表明，某些 zH1 和 zH2 的启动子包含可以对O2以外的因子做出反应的基序。 展开▼

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期刊名称 Plant Physiology

作者
Pietro Ciceri; Silvana Castelli; Massimiliano Lauria; Barbara Lazzari; Annamaria Genga; Loris Bernard; Monica Sturaro; Angelo Viotti;
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年(卷),期 2000(124),1

年度 2000

页码 451–460

总页数 10

原文格式 PDF

正文语种

中图分类人体生理学;

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专利

1. 10kD玉米醇溶蛋白基因克隆及序列分析 [J] . 王广立, 潜忠兴植物学报：英文版 . 1993,第007期

2. Specific combinations of zein genes and genetic backgrounds influence the transcription of the heavy-chain zein genes in maize opaque-2 endosperms [J] . Ciceri P., Lauria M., Lazzari B., Plant physiology . 2000,第1期

机译：玉米醇溶蛋白基因和遗传背景的特定组合影响玉米不透明2胚乳中重链玉米醇溶蛋白基因的转录

3. The ZmbZIP22 Transcription Factor Regulates 27-kD gamma-Zein Gene Transcription during Maize Endosperm Development [J] . Li Chaobin, Yue Yihong, Chen Hanjun, The Plant Cell . 2018,第10期

机译：ZMBZIP22转录因子调节玉米胚乳发育过程中的27-KDγ-ZEIN基因转录

4. Genetic control of the opaque-2 gene and background polygenes over some kernel traits in maize (Zea mays L.) [J] . Xiangyang Lou, Jun Zhu, Quande Zhang, Genetica . 2005,第2a3期

机译：玉米（Zea mays L.）某些籽粒性状对opaque-2基因和背景多基因的遗传控制

5. Gene dosage combinations of sbe1a and ae: Effects on endosperm starch characteristics in the wx background [C] . J. Li, M. Guiltinan, D. Thompson Annual Meeting of the American Association of Cereal Chemists International . 2005

机译：SBE1A和AE的基因剂量组合：对WX背景中胚乳淀粉特性的影响

6. Biochemical and genetic analysis of factors influencing lysinecontent in maize (Zea mays L.) endosperm. [D] . Wang, Xuelu. 2000

机译：影响玉米（Zea mays L.）胚乳赖氨酸含量的因素的生化和遗传分析。

7. The opaque-2 mutation of maize differentially reduces zein gene transcription. [O] . R Kodrzycki, R S Boston, B A Larkins 1989

机译：玉米的opaque-2突变差异地减少了玉米醇溶蛋白基因的转录。

8. Specific Combinations of Zein Genes and Genetic Backgrounds Influence the Transcription of the Heavy-Chain Zein Genes in Maize opaque-2 Endosperms1 [O] . Ciceri, Pietro, Castelli, Silvana, Lauria, Massimiliano, 2000

机译：玉米醇溶蛋白基因和遗传背景的特定组合影响玉米不透明2胚乳中重链玉米醇溶蛋白基因的转录1

9. Cloning of Zein Sequences and an Approach to Zein Genetics [R] . Burr, F. A., Burr, B. 1979

机译：玉米醇溶蛋白序列的克隆及玉米醇溶蛋白遗传学研究

1. 水稻(IR36)基因文库中与玉米醇溶蛋白基因同源克隆的分离 [J] . 夏桂先 . 湖北大学学报（自然科学版） . 1989,第003期

2. 玉米醇溶蛋白γ-zein基因载体构建及亚细胞定位 [J] . 张玉 ,白史且 ,李聪 . 分子植物育种 . 2011,第2期

3. 10kD玉米醇溶蛋白基因的克隆与植物表达载体构建 [J] . 刘晓颖 ,陈芸 ,苗玉青 . 生物技术 . 2007,第4期

4. 转10kD玉米醇溶蛋白基因甘薯蛋白质及农艺性状分析 [J] . 毕瑞明 ,高峰 . 生物技术 . 2007,第3期

5. 逆转录病毒心肌肌凝蛋白a重链基因载体的构建及表达 [C] . 沈茜 ,吕新华 ,曹广文 . 中国科协首届学术年会 . 1999

6. 玉米醇溶蛋白基因的分子进化及其转录因子Opaque2的转录与转录后调控机理 [A] . 李欣欣 . 2019

1. 一种用玉米醇溶蛋白基因RNAi载体提高玉米赖氨酸含量的方法 [P] . 中国专利： CN102517326B . 2013.03.27

2. 一种用玉米醇溶蛋白基因RNAi载体提高玉米赖氨酸含量的方法 [P] . 中国专利： CN102517326A . 2012-06-27

3. rna targeting dna; DNA polynucleotide; recombinant expression vector; genetically modified host cell in vitro; variant site-directed modification polypeptide; chimeric site-directed modification polypeptide; rna polynucleotide; genetically modified cell; transgenic non-human organism whose genome comprises a transgene; composition; site specific modification method of a target DNA; site specific transcriptional modulation method within a target DNA; site-specific modification method in target DNA; method of promoting site-specific cleavage and modification of a target DNA in a cell; method of producing a genetically modified cell in a subject; method of modifying target DNA in a genetically modified cell; kit; target dna target kit; method of selectively modulating transcription of a target DNA into a host cell; isolated nucleic acid; genetically modified host cell; and nucleic acid library [P] . 外国专利： BR112014029441A2 . 2017-06-27

机译：针对dna的rna; DNA多核苷酸;重组表达载体体外转基因宿主细胞;变体定点修饰多肽;嵌合定点修饰多肽; rna多核苷酸;转基因细胞基因组包含转基因的转基因非人类生物;组成;靶DNA的位点特异性修饰方法;靶DNA内的位点特异性转录调节方法;靶DNA的位点特异性修饰方法;促进细胞中靶DNA的位点特异性切割和修饰的方法;在受试者中产生基因修饰的细胞的方法;在转基因细胞中修饰靶DNA的方法;套件目标dna目标试剂盒;选择性调节靶DNA转录到宿主细胞中的方法;分离的核酸转基因宿主细胞;和核酸文库

4. isolated polynucleotide, recombinant dna constructs, vector, process for transformation of a host cell, haploid-inducing maize plant or seed, process for producing haploid-inducing maize plant, process for inducing haploid maize plant lines, processes for identification of a mutant haploid-inducing plant, process for determining the presence, absence or alteration of polynucleotide, mutant haploid-inducing plant, uses of a recombinant DNA, genetic marker of haploid induction in corn plants, process of identifying a corn plant haploid inducer and use of genetic marker [P] . 外国专利： BR112017023922A2 . 2018-07-17

机译：分离的多核苷酸，重组dna构建体，载体，转化宿主细胞的过程，诱导单倍体的玉米植物的过程，诱导单倍体的玉米植物的过程，诱导单倍体玉米的植物系的过程，突变单倍体的鉴定方法诱导植物，确定多核苷酸存在与否的方法，突变单倍体诱导植物，重组DNA的用途，玉米中单倍体诱导的遗传标记，鉴定玉米单倍体诱导剂的过程以及遗传标记的使用

5. Process for the preparation of a lineage of plants genetically transformed plants genetically transformed, corn plants genetically transformed and process for its preparation.Pollen of maize, and apparatus for injecao particles of conduct that lead in living cells DNA [P] . 外国专利： BR8706531A . 1988-07-12

机译：制备转基因植物谱系的方法转基因植物，转基因玉米植物及其制备方法玉米花粉和导致活细胞DNA的行为的注射装置

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Specific Combinations of Zein Genes and Genetic Backgrounds Influence the Transcription of the Heavy-Chain Zein Genes in Maize opaque-2 Endosperms

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