• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Plant Physiology >INDETERMINATE SPIKELET1 Recruits Histone Deacetylase and a Transcriptional Repression Complex to Regulate Rice Salt Tolerance
【2h】

INDETERMINATE SPIKELET1 Recruits Histone Deacetylase and a Transcriptional Repression Complex to Regulate Rice Salt Tolerance

机译：不确定小穗1招募组蛋白脱乙酰基酶和转录抑制复合物以调节水稻的耐盐性

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Perception and transduction of salt stress signals are critical for plant survival, growth, and propagation. Thus, identification of components of the salt stress-signaling pathway is important for rice (Oryza sativa) molecular breeding of salt stress resistance. Here, we report the identification of an apetala2/ethylene response factor transcription factor INDETERMINATE SPIKELET1 (IDS1) and its roles in the regulation of rice salt tolerance. By genetic screening and phenotype analysis, we demonstrated that IDS1 conferred transcriptional repression activity and acted as a negative regulator of salt tolerance in rice. To identify potential downstream target genes regulated by IDS1, we conducted chromatin immunoprecipitation (ChIP) sequencing and ChIP-quantitative PCR assays and found that IDS1 may directly associate with the GCC-box-containing motifs in the promoter regions of abiotic stress-responsive genes, including LEA1 (LATE EMBRYOGENESIS ABUNDANT PROTEIN1) and SOS1 (SALT OVERLY SENSITIVE1), which are key genes regulating rice salt tolerance. IDS1 physically interacted with the transcriptional corepressor topless-related 1 and the histone deacetylase HDA1, contributing to the repression of LEA1 and SOS1 expression. Analyses of histone H3 acetylation status and RNA polymerase II occupation on the promoters of LEA1 and SOS1 further defined the molecular foundation of the transcriptional repression activity of IDS1. Our findings illustrate an epigenetic mechanism by which IDS1 modulates salt stress signaling as well as salt tolerance in rice.
机译：盐胁迫信号的感知和转导对于植物存活，生长和繁殖至关重要。因此，鉴定盐胁迫信号通路的成分对于水稻（Oryza sativa）抗盐胁迫分子育种很重要。在这里，我们报告鉴定apetala2 /乙烯反应因子转录因子INDETERMINATE SPIKELET1（IDS1）及其在调节水稻盐耐性中的作用。通过遗传筛选和表型分析，我们证明IDS1赋予转录抑制活性，并作为水稻耐盐性的负调控因子。为了确定IDS1调控的潜在下游靶基因，我们进行了染色质免疫沉淀（ChIP）测序和ChIP定量PCR分析，发现IDS1可能与非生物胁迫响应基因启动子区域中含有GCC-box的基序直接相关，包括LEA1（晚期胚发生丰富蛋白1）和SOS1（盐过度敏感1），它们是调节水稻耐盐性的关键基因。 IDS1与转录共加压因子裸照相关1和组蛋白脱乙酰基酶HDA1物理相互作用，从而有助于抑制LEA1和SOS1的表达。对LEA1和SOS1启动子的组蛋白H3乙酰化状态和RNA聚合酶II占领的分析进一步确定了IDS1转录抑制活性的分子基础。我们的发现说明了IDS1调节水稻盐胁迫信号传导和耐盐性的表观遗传机制。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号