Reconstitution of purified brown adipose tissue mitochondria uncoupling protein: demonstration of separate identity of nucleotide binding and proton translocation sites by chemical probes.

机译：纯化的棕色脂肪组织线粒体解偶联蛋白的重建：通过化学探针证明核苷酸结合和质子转运位点的独立性。

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摘要

The reaction of two arginine-modifying reagents, phenylglyoxal and 2,3-butanedione, with a highly purified uncoupling protein of brown adipose tissue mitochondria decreased the GDP binding to the uncoupling protein. This inhibition was irreversible and dependent on time and concentration of the reagent. Complete inhibition of GDP binding by both reagents establishes that arginine is one of the critical amino acid residues involved in the binding of GDP. Reconstitution of the uncoupling protein (both unmodified and modified) into phospholipid vesicles by our procedure showed no effect of phenylglyoxal modification on the H+ conductance, thus demonstrating that the proton translocation site is structurally different and distinct from the GDP binding site.

机译：两种精氨酸修饰剂苯乙二醛和2，3-丁二酮与高度纯化的棕色脂肪组织线粒体的解偶联蛋白的反应降低了GDP与解偶联蛋白的结合。这种抑制是不可逆的，并且取决于试剂的时间和浓度。两种试剂对GDP结合的完全抑制建立了精氨酸是参与GDP结合的关键氨基酸残基之一。通过我们的程序将解偶联蛋白（未修饰的和修饰的）重建为磷脂囊泡，未显示苯乙二醛修饰对H +电导的影响，因此表明质子转运位点在结构上与GDP结合位点不同。

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期刊名称 Proceedings of the National Academy of Sciences of the United States of America
作者
S S Katiyar; E Shrago;
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年(卷),期 1989(86),8
年度 1989
页码 2559–2562
总页数 4
原文格式 PDF
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专利

1. TWO-STAGE NUCLEOTIDE BINDING MECHANISM AND ITS IMPLICATIONS TO H+ TRANSPORT INHIBITION OF THE UNCOUPLING PROTEIN FROM BROWN ADIPOSE TISSUE MITOCHONDRIAL [J] . Huang SG, Klingenberg M Biochemistry . 1996,第24期

机译：棕色脂肪组织线粒体解偶联蛋白的两阶段核苷酸结合机理及其对H +转运的抑制作用
2. Acute effects of a β-adrenoceptor agonist (BRL 26830A) on rat brown-adipose-tissue mitochondria. Increased GDP binding and GDP-sensitive proton conductance without changes in the concentration of uncoupling protein [J] . J R Arch, P Trayhurn, R E Milner, The biochemical journal . 1988,第3期

机译：β肾上腺素受体激动剂（BRL 26830A）对大鼠褐色脂肪组织线粒体的急性作用。增加GDP结合力和GDP敏感的质子电导率，而未改变解偶联蛋白的浓度
3. Complete nucleotide and derived amino acid sequence of cDNA encoding the mitochondrial uncoupling protein of rat brown adipose tissue: lack of a mitochondrial targeting presequence [J] . Gerhard E. Gerber, Hasmukh V. Patel, Karl B. Freeman, Nucleic acids research . 1986,第10期

机译：编码大鼠棕色脂肪组织线粒体解偶联蛋白的cDNA的完整核苷酸和衍生氨基酸序列：缺乏线粒体靶向序列
4. Proteome-Wide Discovery and Characterizations of Nucleotide-Binding Proteins With Affinity-Labeled Chemical Probes. [D] . Xiao, Yongsheng. 2014

机译：使用亲和标签的化学探针对蛋白质组蛋白进行核苷酸结合蛋白的发现和表征。
5. Acute effects of a beta-adrenoceptor agonist (BRL 26830A) on rat brown-adipose-tissue mitochondria. Increased GDP binding and GDP-sensitive proton conductance without changes in the concentration of uncoupling protein. [O] . R E Milner, S Wilson, J R Arch, 1988

机译：β肾上腺素受体激动剂（BRL 26830A）对大鼠棕色脂肪组织线粒体的急性作用。增加了GDP结合力和GDP敏感性质子电导而未改变解偶联蛋白的浓度。
6. Reconstitution of purified brown adipose tissue mitochondria uncoupling protein: demonstration of separate identity of nucleotide binding and proton translocation sites by chemical probes. [O] . Katiyar, S S, Shrago, E 1989

机译：纯化的棕色脂肪组织线粒体解偶联蛋白的重建：通过化学探针证明核苷酸结合和质子转运位点的独立性。

Reconstitution of purified brown adipose tissue mitochondria uncoupling protein: demonstration of separate identity of nucleotide binding and proton translocation sites by chemical probes.

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