RecA protein stimulates homologous recombination in plants.

机译：RecA蛋白刺激植物中的同源重组。

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摘要

A number of RecA-like proteins have been found in eukaryotic organisms. We demonstrate that the prokaryotic recombination protein RecA itself is capable of interacting with genomic homologous DNA in somatic plant cells. Resistance to the DNA crosslinking agent mitomycin C requires homologous recombination as well as excision repair activity. Tobacco protoplasts expressing a nucleus-targeted RecA protein were at least three times as efficient as wild-type cells in repairing mitomycin C-induced damage. Moreover, homologous recombination at a defined locus carrying an endogenous nuclear marker gene was stimulated at least 10-fold in transgenic plant cells expressing nucleus-targeted RecA. The increase in resistance to mitomycin C and the stimulation of intrachromosomal recombination demonstrate that Escherichia coli RecA protein is functional in genomic homologous recombination in plants, especially when targeted to the plant nucleus.

机译：在真核生物中发现了许多类似RecA的蛋白质。我们证明原核重组蛋白RecA本身能够与体植物细胞中的基因组同源DNA相互作用。对DNA交联剂丝裂霉素C的抗性需要同源重组以及切除修复活性。表达核靶向RecA蛋白的烟草原生质体在修复丝裂霉素C诱导的损伤方面的效率至少是野生型细胞的三倍。此外，携带表达内源性核标记基因的特定基因座处的同源重组在表达核靶向RecA的转基因植物细胞中被刺激了至少10倍。对丝裂霉素C的抗性增加和染色体内重组的刺激表明，大肠杆菌RecA蛋白在植物的基因组同源重组中起作用，特别是当靶向植物核时。

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期刊名称 Proceedings of the National Academy of Sciences of the United States of America
作者
B Reiss; M Klemm; H Kosak; J Schell;
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年(卷),期 1996(93),7
年度 1996
页码 3094–3098
总页数 5
原文格式 PDF
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专利

1. Overexpression of bacterial RecA protein stimulates homologous recombination in somatic mammalian cells. [J] . Shcherbakova OG, Lanzov VA, Ogawa H, Mutation Research: International Journal on Mutagenesis, Chromosome Breakage and Related Subjects . 2000,第1期

机译：细菌RecA蛋白的过度表达会刺激哺乳动物体细胞中的同源重组。
2. RecA protein stimulates homologous recombination in plants [J] . Bernd Reiss, Manfred Klemm, Hans Kosak Proceedings of the National Academy of Sciences of the United States of America . 1996,第7期

机译：RecA蛋白刺激植物中的同源重组
3. Investigation of mycobacterial recA function: protein introns in the RecA of pathogenic mycobacteria do not affect competency for homologous recombination. [J] . Frischkorn K, Sander P, Scholz M, Molecular Microbiology . 1998,第5期

机译：分枝杆菌recA功能的研究：致病性分枝杆菌的RecA中的蛋白质内含子不影响同源重组的能力。
4. Comparative genomics revealed a novel DNA-binding regulatory protein involved in homologous recombination in bacteria [C] . Yang Gao, Yan Zhang 2011 IEEE International Conference on Systems Biology . 2011

机译：比较基因组学揭示了一种新型的DNA结合调节蛋白，参与细菌的同源重组
5. The role of homologous recombination in the production of debilitating mitochondrial DNA rearrangements in plants. [D] . Palucki, Elaine Marie. 2003

机译：同源重组在破坏植物线粒体DNA重排中的作用。
6. Mechanism of the concerted action of recA protein and helix-destabilizing proteins in homologous recombination. [O] . K Muniyappa, S L Shaner, S S Tsang, 1984

机译：recA蛋白和螺旋不稳定蛋白在同源重组中协同作用的机制。
7. RecA protein stimulates homologous recombination in plants. [O] . Reiss, B, Klemm, M, Kosak, H, 1996

机译：RecA蛋白刺激植物中的同源重组。
8. Characterization of the 'Pseudomonas aeruginosa recA' Analog and Its Protein Product: 'rec-102' is a Mutant Allele of the 'P. aeruginosa' PAO 'recA' Gene [R] . Kokjohn, T. A. , Miller, R. V. 1987

机译：'铜绿假单胞菌reca'类似物及其蛋白质产物的表征：'rec-102'是'p'的突变等位基因。铜绿假单胞菌'paO'reca'基因

RecA protein stimulates homologous recombination in plants.

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