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Characterization of the 'Pseudomonas aeruginosa recA' Analog and Its Protein Product: 'rec-102' is a Mutant Allele of the 'P. aeruginosa' PAO 'recA' Gene

机译:'铜绿假单胞菌reca'类似物及其蛋白质产物的表征:'rec-102'是'p'的突变等位基因。铜绿假单胞菌'paO'reca'基因

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Cloned was a 2.3 kilobase pair fragment of the Pseudomonas aeruginosa PAO chromosome which is capable of complementing recA mutations of Escherichia coli. The recA-complementing activity was further localized to a 1.5 kilobase pair PvuII-HindIII fragment. The direction of transcription was determined. Southern analysis under conditions of high stringency indicated that DNA sequence homology is shared by the E. coli recA gene and the P. aeruginosa recA analogue. The cloned recA analogue was shown to restore resistance to methyl methane-sulfonate, nitrofurantoin and ultraviolet irradiation to E coli recA mutants. (Copyright (c) 1987, American Society for Microbiology.)

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