A calcium responsive element that regulates expression of two calcium binding proteins in Purkinje cells

机译：钙反应元件调节浦肯野细胞中两种钙结合蛋白的表达

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Calbindin D28 encodes a calcium binding protein that is expressed in the cerebellum exclusively in Purkinje cells. We have used biolistic transfection of organotypic slices of P12 cerebellum to identify a 40-bp element from the calbindin promoter that is necessary and sufficient for Purkinje cell specific expression in this transient in situ assay. This element (PCE1) is also present in the calmodulin II promoter, which regulates expression of a second Purkinje cell Ca²⁺ binding protein. Expression of high levels of exogenous calbindin or calretinin decreased transcription mediated by PCE1 in Purkinje cells 2.5- to 3-fold, whereas the presence of 1 μM ionomycin in the extracellular medium increased expression. These results demonstrate that PCE1 is a component of a cell-specific and Ca²⁺-sensitive transcriptional regulatory mechanism that may play a key role in setting the Ca²⁺ buffering capacity of Purkinje cells.

机译：钙结合蛋白D28编码钙结合蛋白，其仅在浦肯野细胞中在小脑中表达。我们已经使用P12小脑器官型切片的生物弹射转染从calbindin启动子中鉴定出40 bp的元件，该元件对于在此瞬时原位测定中浦肯野细胞特异性表达是必需的和充分的。该元素（PCE1）也存在于钙调蛋白II启动子中，该启动子调节第二浦肯野细胞Ca ^{2 + 结合蛋白的表达。高水平外源钙结合蛋白或降钙素的表达使浦肯野细胞中PCE1介导的转录降低了2.5到3倍，而细胞外培养基中存在1μM的离子霉素则增加了表达。这些结果表明，PCE1是细胞特异性且对Ca ^{2 + 敏感的转录调控机制的组成部分，在调节Ca ^{2 + 缓冲能力中可能起关键作用浦肯野细胞。}}}

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期刊名称 Proceedings of the National Academy of Sciences of the United States of America
作者
Donald B. Arnold; Nathaniel Heintz;
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作者单位

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年(卷),期 1997(94),16
年度 1997
页码 8842–8847
总页数 6
原文格式 PDF
正文语种
中图分类
关键词
transcription biolistic transfection Casup2+/sup responses calbindin calmodulin II;

机译：转录;基因枪转染;Ca sup 2 + / sup反应;钙结合蛋白;钙调节蛋白II;

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专利

1. A calcium responsive element that regulates expression of two calcium binding proteins in Purkinje cells [J] . Donald B. Arnold, Nathaniel Heintz Proceedings of the National Academy of Sciences of the United States of America . 1997,第16期

机译：钙反应元件，调节浦肯野细胞中两种钙结合蛋白的表达
2. Altered expression of calcium- and apoptosis-regulating proteins in multiple system atrophy Purkinje cells. [J] . Wullner U, Weller M, Kornhuber J, Movement disorders . 2000,第2期

机译：钙和凋亡调节蛋白在多系统萎缩浦肯野细胞中表达的改变。
3. The neuronal EF-hand calcium-binding protein visinin-like protein-3 is expressed in cerebellar Purkinje cells and shows a calcium-dependent membrane association. [J] . Spilker C, Richter K, Smalla KH, Neuroscience: An International Journal under the Editorial Direction of IBRO . 2000,第1期

机译：神经元EF手钙结合蛋白visinin样蛋白3在小脑浦肯野细胞中表达，并显示钙依赖性膜结合。
4. REGULATORY EXPRESSION OF FOXO3A PROTEINS IN DIFFERENT SIZE OF FOLLICLES AND CHANGED CONCENTRATIONS OF INTRACELLULAR CALCIUM DURING MATURATION OF GRANULOSA CELLS IN VITRO IN THE PIG [C] . WANG Qian, rnWU Ning, rnLUO Peng Current Status and Innovation in Animal Reproductive Biotechnology and Stem Cells . 2008

机译：猪粒状细胞体外成熟过程中不同大小卵泡中FOXO3A蛋白的表达及细胞内钙浓度的变化
5. Effects of milk proteins on intracellular calcium ion concentration and stallion sperm binding to zonae pellucidae. [D] . Coutinho da Silva, Marco Antonio. 2005

机译：牛奶蛋白对细胞内钙离子浓度和种马精子结合至透明带的影响。
6. Calcium/calmodulin-dependent protein kinase II regulates cyclooxygenase-2 expression and prostaglandin E2 production by activating cAMP-response element-binding protein in rat peritoneal macrophages [O] . Xueyuan Zhou, Junying Li, Wenxiu Yang 2014

机译：钙/钙调蛋白依赖性蛋白激酶II通过激活大鼠腹膜巨噬细胞中的cAMP反应元件结合蛋白来调节环氧合酶2的表达和前列腺素E2的产生
7. Calcium and cAMP Signals Differentially Regulate cAMP-responsive Element-binding Protein Function via a Rap1-Extracellular Signal-regulated Kinase Pathway [O] . Savraj S. Grewal, Daniel M. Fass, Hong Yao, 2000

机译：钙和阵营信号通过Rap1-Uthroblular信号调节激酶途径差异调节阵营响应元件结合蛋白质功能
8. Repair of Nerve Cell Membrance Damage by Calcium-Dependent, Membrane- Binding Proteins. [R] . C. E. Creutz 2013

机译：钙依赖性膜结合蛋白修复神经细胞膜损伤。

A calcium responsive element that regulates expression of two calcium binding proteins in Purkinje cells

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