Locating and identifying posttranslational modifications by in-source decay during MALDI-TOF mass spectrometry.

机译：通过MALDI-TOF质谱分析中的源内衰减来定位和识别翻译后修饰。

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摘要

A technique is described for identifying and locating posttranslational modifications (PTMs) in peptides and proteins of known sequence by interpretation of c(n) ion signals generated by in-source decay during delayed ion extraction in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Sites of phosphorylation in seven synthetic peptides were determined, as was the location of both the heme group and N,N,N-trimethyllysine in yeast cytochrome c. A semi-automated data analysis process facilitates the identification of segments of the sequence on each side of the PTM, permitting its placement at the junction of the segments and definition of the added mass. A graphical display facilitates illustration of both the location and mass of the PTM.

机译：描述了一种通过在基质辅助激光解吸/电离时间的延迟离子提取过程中通过源内衰减产生的c（n）离子信号的解释来识别和定位已知序列的肽和蛋白质中的翻译后修饰（PTM）的技术。飞行质谱。确定了七个合成肽中的磷酸化位点，以及血红素基团和N，N，N-三甲基赖氨酸在酵母细胞色素c中的位置。半自动数据分析过程有助于在PTM的每一侧识别序列的片段，从而允许将其放置在片段的交界处并定义增加的质量。图形显示有助于说明PTM的位置和质量。

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期刊名称 Protein Science : A Publication of the Protein Society
作者
J. J. Lennon; K. A. Walsh;
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作者单位

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年(卷),期 1999(8),11
年度 1999
页码 2487–2493
总页数 7
原文格式 PDF
正文语种
中图分类分子生物学;
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专利

1. Locating and identifying posttranslational modifications by in-source decay during MALDI-TOF mass spectrometry. [J] . Lennon JJ, Walsh KA Protein Science: A Publication of the Protein Society . 1999,第11期

机译：通过MALDI-TOF质谱分析中的源内衰减来定位和识别翻译后修饰。
2. Posttranslational Modifications in Lens Fiber Connexins Identified by Off-Line-HPLC MALDI-Quadrupole Time-of-Flight Mass Spectrometry. [J] . Shearer D, Ens W, Standing K, Investigative ophthalmology & visual science . 2008,第4期

机译：脱机HPLC MALDI-四极杆飞行时间质谱法鉴定的镜片纤维连接蛋白的翻译后修饰。
3. Matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry. A comparison of fragmentation patterns of linear dextran obtained by in-source decay, post-source decay and collision-induced dissociation and the stability of linear and c [J] . Sajid Bashir, Anastassios E. Giannakopulos, Peter J. Derrick, European journal of mass spectrometry . 2004,第1期

机译：基质辅助激光解吸/电离飞行时间质谱。通过源内衰减，源后衰减和碰撞诱导的解离获得的线性右旋糖酐的碎裂模式比较以及线性和c的稳定性
4. New improvements and understandings of In-Source Decay (ISD) of peptides in MALDI-TOF Mass Spectrometry [C] . Kevin Demeure, Valerie Gabelica, Frederic Rosu, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2009

机译：MALDI-TOF质谱中肽源衰减（ISD）的新改进和谅解
5. Study of posttranslational modifications in proteins and noncovalent interactions in small models by mass spectrometry. [D] . Zickus, Michael A. 2010

机译：通过质谱研究小模型中蛋白质的翻译后修饰和非共价相互作用。
6. Identification of the posttranslational modifications of bovine lens alpha B-crystallins by mass spectrometry. [O] . J. B. Smith, Y. Sun, D. L. Smith, 1992

机译：通过质谱鉴定牛晶状体αB-晶状体蛋白的翻译后修饰。
7. Locating and identifying posttranslational modifications by in-source decay during MALDI-TOF mass spectrometry. [O] . Lennon, J. J., Walsh, K. A. 2008

机译：通过MALDI-TOF质谱分析中的源内衰减来定位和识别翻译后修饰。

Locating and identifying posttranslational modifications by in-source decay during MALDI-TOF mass spectrometry.

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