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首页> 美国卫生研究院文献>Tissue Engineering and Regenerative Medicine >Fabrication and Characterization of Polyphosphazene/Calcium Phosphate Scaffolds Containing Chitosan Microspheres for Sustained Release of Bone Morphogenetic Protein 2 in Bone Tissue Engineering
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Fabrication and Characterization of Polyphosphazene/Calcium Phosphate Scaffolds Containing Chitosan Microspheres for Sustained Release of Bone Morphogenetic Protein 2 in Bone Tissue Engineering

机译：含壳聚糖微球的聚磷腈/磷酸钙支架的制备与表征用于骨组织工程中骨形态发生蛋白2的持续释放

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Bone morphogenetic protein 2 has a major role in promoting bone regeneration in tissue engineering scaffolds. Growth factor release rate is a remaining crucial problem in these systems. The aim of this study was to fabricate and characterize a novel calcium phosphate/polyphosphazenes porous scaffold for the sustained release of bone morphogenetic protein 2 in bone tissue engineering. Polyphosphazenes were substituted with 2-dimethylaminoethanol and evaluated by GPC, NMR, and in vitro degradation. Calcium phosphate porous samples were prepared from hydroxyapatite nanoparticles and naphthalene using the sintering method at 1250 °C before being composited with poly(dimethylaminoethanol)phosphazenes containing chitosan microspheres loaded with bone morphogenetic protein 2. The characteristics and biodegradability of the product were evaluated by SEM, XRD, and in vitro degradation. Moreover, the release rate and mechanical properties of the scaffolds were investigated. The release behavior was found to be sustained since the scaffolds had been fabricated from polyphosphazenes with a low degradation rate. The release rates of the scaffolds were observed to increase with increasing chitosan microspheres content from 10 to 30%. The bioactivity of the scaffolds depended on the release rate of growth factor while bone morphogenetic protein 2 was able to induce an osteoblast proliferation. The results of cell adhesion and cell viability tests showed that scaffolds displayed a non-toxic behavior and western blot analyses confirmed that the scaffolds loaded with growth factor increased the osteogenic differentiation potential of cells when compared with scaffolds alone. These results demonstrate that these scaffolds can be successfully utilized in bone tissue engineering.
机译：骨形态发生蛋白2在促进组织工程支架中的骨再生中起主要作用。在这些系统中，生长因子释放速率仍然是关键问题。这项研究的目的是制造和表征新型的磷酸钙/聚磷腈多孔支架，以在骨组织工程中持续释放骨形态发生蛋白2。用2-二甲基氨基乙醇取代聚磷腈，并通过GPC，NMR和体外降解进行评估。在1250°C下用烧结方法从羟基磷灰石纳米颗粒和萘制备磷酸钙多孔样品，然后将其与载有骨形态发生蛋白2的壳聚糖微球的聚（二甲基氨基乙醇）磷腈复合。通过SEM评估产品的特性和生物降解性， XRD和体外降解。此外，研究了支架的释放速率和机械性能。由于支架是由降解速率低的聚磷腈制成的，因此发现释放行为得以维持。观察到支架的释放速率随着壳聚糖微球含量从10％到30％的增加而增加。支架的生物活性取决于生长因子的释放速率，而骨形态发生蛋白2能够诱导成骨细胞增殖。细胞粘附和细胞活力测试的结果表明，支架表现出无毒行为，蛋白质印迹分析证实与单独的支架相比，负载有生长因子的支架增加了细胞的成骨分化潜能。这些结果表明，这些支架可以成功地用于骨组织工程中。

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