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首页> 美国卫生研究院文献>Tissue Engineering and Regenerative Medicine >Differentiation Potential of Breast Milk-Derived Mesenchymal Stem Cells into Hepatocyte-Like Cells
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Differentiation Potential of Breast Milk-Derived Mesenchymal Stem Cells into Hepatocyte-Like Cells

机译：母乳来源的间充质干细胞向肝细胞样细胞的分化潜能

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摘要

Human breast milk stem cells (hBSCs) contain a population of cells with the ability to differentiate into various cell lineages for cell therapy applications. The current study examined the differentiation potential of hBSCs into hepatocytes-like cells. The cells were isolated from the breast milk and were treated with hepatogenic medium containing hepatocyte growth factor, insulin-like growth factor and dexamethasone for 7 days subsequently; Oncostatin M was added to the culture media. RT-PCR and immunocytochemistry were performed to detect the hepatogenic markers. The glycogen storage and the ability of the cells to absorb and release indocynanin green were also tested. The data showed that most of the differentiated cells formed cell aggregates after the 30th day, with more cells accumulated to form spheroids. RT-PCR revealed the expression of the hepatic nuclear factor, albumin, cytokeratin 18 and 19, cytochrome P2B6, glucose-6-phospahtase and claudin. The functional assays also showed glycogen storage and omission of indicynine green. Our study demonstrated hBSCs are novel population that can differentiate into hepatocyte-like cells.
机译：人母乳干细胞（hBSC）包含大量细胞，这些细胞具有分化为细胞疗法应用的各种细胞谱系的能力。当前的研究检查了hBSCs分化为肝细胞样细胞的潜力。从母乳中分离细胞，然后用含有肝细胞生长因子，胰岛素样生长因子和地塞米松的肝生成培养基处理7天；将制抑素M加入培养基中。进行RT-PCR和免疫细胞化学检测肝原性标志物。还测试了糖原的储存以及细胞吸收和释放吲哚南绿的能力。数据显示，大多数分化的细胞在第30天后形成细胞聚集体，更多的细胞积累形成球状体。 RT-PCR揭示了肝核因子，白蛋白，细胞角蛋白18和19，细胞色素P2B6，葡萄糖-6-磷酸酶和claudin的表达。功能测定还显示了糖原的储存和靛蓝绿色的省略。我们的研究表明，hBSCs是可以分化为肝细胞样细胞的新型种群。

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