• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Toxins >An Imaging Surface Plasmon Resonance Biosensor Assay for the Detection of T-2 Toxin and Masked T-2 Toxin-3-Glucoside in Wheat
【2h】

An Imaging Surface Plasmon Resonance Biosensor Assay for the Detection of T-2 Toxin and Masked T-2 Toxin-3-Glucoside in Wheat

机译：成像表面等离子体共振生物传感器检测小麦中T-2毒素和掩盖的T-2毒素-3-糖苷的方法

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A sensitive, rapid, and reproducible imaging surface plasmon resonance (iSPR) biosensor assay was developed to detect T-2 toxin and T-2 toxin-3-glucoside (T2-G) in wheat. In this competitive assay, an amplification strategy was used after conjugating a secondary antibody (Ab2) with gold nanoparticles. Wheat samples were extracted with a methanol/water mixture (80:20 v/v), then diluted with an equal volume of primary antibody (Ab1) for analysis. Matrix-matched calibration curves were prepared to determine T-2 toxin and T2-G. Recovery studies were conducted at three spiking levels in blank wheat. Mean recoveries ranged from 86 to 90%, with relative standard deviations for repeatability (RSDr) of less than 6%. Limits of detection were 1.2 ng/mL of T-2 toxin and 0.9 ng/mL of T2-G, equivalent to their levels in wheat, of 48 and 36 µg/kg, respectively. The developed iSPR assay was rapid and provided enough sensitivity for the monitoring of T-2 toxin/T2-G in wheat. This is the first iSPR assay useful for detecting the “masked” T2-G in wheat.
机译：开发了一种灵敏，快速且可重现的成像表面等离子体共振（iSPR）生物传感器测定法，用于检测小麦中的T-2毒素和T-2毒素-3-葡萄糖苷（T2-G）。在该竞争性测定中，将二抗（Ab2）与金纳米颗粒结合后使用了扩增策略。用甲醇/水混合物（80:20 v / v）提取小麦样品，然后用等体积的一抗（Ab1）稀释以进行分析。制备基质匹配的校准曲线以确定T-2毒素和T2-G。在空白小麦的三个穗水平上进行了恢复研究。平均回收率在86％到90％之间，可重复性的相对标准偏差（RSDr）小于6％。检出限分别为1.2 ng / mL的T-2毒素和0.9 ng / mL的T2-G，相当于小麦中的水平，分别为48和36 µg / kg。所开发的iSPR测定快速且为监测小麦中的T-2毒素/ T2-G提供了足够的灵敏度。这是第一个用于检测小麦中“掩盖”的T2-G的iSPR分析。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号