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首页> 美国卫生研究院文献>Toxins >A QuEChERS-Based Liquid Chromatography-Tandem Mass Spectrometry Method for the Simultaneous Determination of Nine Zearalenone-Like Mycotoxins in Pigs
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A QuEChERS-Based Liquid Chromatography-Tandem Mass Spectrometry Method for the Simultaneous Determination of Nine Zearalenone-Like Mycotoxins in Pigs

机译：基于QuEChERS的液相色谱-串联质谱法同时测定猪中的九种玉米赤霉烯酮样真菌毒素

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摘要

The determination of zearalenone (ZEN) and its derivatives as biomarkers in animal tissues or organs plays an important role in mycotoxin monitoring and can promote effective exposure assessment. A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the simultaneous quantification of nine ZEN-like mycotoxins, including three glucuronides in different pig tissues (heart, liver, spleen and muscle) was developed and validated in this study. Tissue samples were extracted using a quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction and clean-up procedure, and analyzed by LC-MS/MS in multiple reaction monitoring (MRM) mode. Dynamic linear ranges for each target analyte were determined with R2 between 0.916 and 0.999. The LODs of the six ZENs were achieved in the range of 0.5–1 ng/g and the LOQs varied from 1 ng/g to 2 ng/g. The satisfying intra-day and inter-day reproducibility (both RSDr and RSDR < 20%) indicated a good stability of this method. The recoveries of the nine target analytes were in the range of 70–110%. The validation results showed that this LC-MS/MS method coupled with QuEChERS sample pretreatment is effective and suitable for the simultaneous quantitation of ZEN metabolites in pigs. It has been applied to analysis of the pig tissues in this research and can be also adapted for samples in the mycotoxin research field.
机译：玉米赤霉烯酮（ZEN）及其衍生物作为动物组织或器官中生物标志物的测定在霉菌毒素监测中起着重要作用，并且可以促进有效的暴露评估。开发了一种液相色谱-串联质谱（LC-MS / MS）方法，用于同时定量分析不同猪组织（心脏，肝脏，脾脏和肌肉）中的9种ZEN样真菌毒素，包括3种葡糖醛酸苷。使用快速，简便，便宜，有效，坚固耐用且安全的（QuEChERS）提取和净化程序提取组织样品，并通过LC-MS / MS在多反应监测（MRM）模式下进行分析。测定每个目标分析物的动态线性范围，R 2 在0.916至0.999之间。六个ZEN的LOD在0.5-1 ng / g范围内，LOQ从1 ng / g到2 ng / g不等。令人满意的日内和日间重现性（RSDr和RSDR均<20％）表明该方法具有良好的稳定性。九种目标分析物的回收率在70％至110％之间。验证结果表明，这种LC-MS / MS方法与QuEChERS样品预处理相结合是有效的，适用于同时定量猪中ZEN代谢物。在本研究中，它已被用于分析猪的组织，也可用于霉菌毒素研究领域的样品。

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