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首页> 美国卫生研究院文献>Journal of Traditional and Complementary Medicine >In vitro and in vivo antileishmanial effects of aloe-emodin on Leishmania major
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In vitro and in vivo antileishmanial effects of aloe-emodin on Leishmania major

机译：芦荟大黄素对利什曼原虫的体外和体内抗衰老作用

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Cutaneous leishmaniasis is a common parasitic disease that is endemic in some parts of Iran. The drugs of choice used for leishmaniasis therapy are associated with a risk of recurrence and serious adverse effects. Therefore, finding a safe and effective treatment is of great importance. In the present study, the effect of aloe-emodin on the growth of Leishmania major amastigotes was evaluated under in vitro conditions. In addition, the efficacy of a topical of aloe-emodin ointment was investigated in BALB/c mice with cutaneous leishmanial ulcers. Different concentrations (40 μg/mL, 80 μg/mL, 120 μg/mL, and 160 μg/mL) of aloe-emodin were tested on Leishmania amastigotes twice: 24 hours and 48 hours. The induced apoptosis and necrotic effects of two concentrations (40 μg/mL and 120 μg/mL) of aloe-emodin on promastigotes were investigated by flow cytometry. Under the in vivo condition, aloe-emodin ointment efficacy was evaluated at two concentrations (i.e., 0.1% and 1%). Serum indicator factors of the test and control groups were tested to evaluate the toxic effects of this compound on the liver and kidney. Results showed that aloe-emodin inhibited the growth of Leishmania amastigotes and induced apoptosis in promastigotes. Topical application of aloe-emodin ointment likewise reduced the ulcer size. No significant differences in biochemical analysis were observed between the control and treated groups. In conclusion, aloe-emodin showed antileishmanial effects under in vitro and in vivo conditions and may be used in clinical trials.
机译：皮肤利什曼病是一种常见的寄生虫病，在伊朗某些地区是地方性流行病。用于利什曼病治疗的所选药物具有复发风险和严重的不良反应。因此，寻找安全有效的治疗方法非常重要。在本研究中，在体外条件下评估了芦荟大黄素对利什曼原虫主要变形虫生长的影响。另外，在患有皮肤利什曼病的BALB / c小鼠中研究了芦荟-大黄素软膏局部用药的功效。在利什曼原虫amastigotes上测试了不同浓度的芦荟大黄素（40μg/ mL，80μg/ mL，120μg/ mL和160μg/ mL）：24小时和48小时。通过流式细胞术研究了芦荟大黄素两种浓度（40μg/ mL和120μg/ mL）对前鞭毛体的诱导凋亡和坏死作用。在体内条件下，以两种浓度（即0.1％和1％）评估芦荟大黄素软膏的功效。测试了测试组和对照组的血清指示剂，以评估该化合物对肝脏和肾脏的毒性作用。结果表明，芦荟大黄素抑制了利什曼原虫的生长并诱导了前鞭毛体的凋亡。芦荟大黄素软膏的局部应用同样可以减少溃疡的大小。对照组和治疗组之间在生化分析上没有观察到显着差异。总之，芦荟大黄素在体外和体内条件下均表现出抗衰老作用，可用于临床试验。

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