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首页> 美国卫生研究院文献>The Journal of Physiology >The utilization of glucose and production of lactate by in vitro preparations of rat small intestine: effects of vascular perfusion.
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The utilization of glucose and production of lactate by in vitro preparations of rat small intestine: effects of vascular perfusion.

机译：大鼠小肠体外制剂对葡萄糖的利用和乳酸的产生：血管灌注的影响。

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1. The rate of metabolism of glucose to lactate has been measured in a number of non-vascularly perfused preparations of rat jejunum in vitro. The glucose and lactate metabolism was measured simultaneously and under conditions such that the uptake of glucose and the appearance of lactate were linearly related to time. 2. It is found that there is no difference between the rates at which rings of rat jejunum utilize glucose during the first 45 min of anaerobic or aerobic incubation. During the first 15 min of incubation between 60-70% of the metabolized glucose was converted to lactate under aerobic conditions; this value increased to 80-90% during the subsequent 30 min. During the period 0-15 min of incubation, lactate production was found to be higher under anaerobic than under aerobic conditions but after this initial period the rate of lactate production was the same under aerobic and anaerobic conditions. 3. For segments of rat jejunum, maintained in vitro by the recirculation of nutrient fluid through the intestinal lumen, neither the rate of production of lactate, nor the utilization of glucose, was stimulated if the preparation was maintained under anaerobic rather than aerobic conditions. The direct delivery of gas into the intestinal lumen in the form of a stream of bubbles (segmented circulation) reduced both the utilization of glucose and the production of lactate under aerobic conditions. However, not effect on glucose metabolism was observed under anaerobic conditions. The finding of a Pasteur effect with the segmented-circulated preparation, but not with the simple recirculated preparation, is associated with lower rate of aerobic lactate production in the former preparation. Reasons are given for supposing that under conditions of segmented circulation, the luminal compartment is better stirred, thereby increasing access of O2 to the tissue. 4. A preparation of rat small intestine perfused through the vascular bed is described. With this preparation the rate of glucose utilization is significantly lower than that for recirculated preparations and the rate of lactate production is substantially less than that of the other preparations studied. 5. With the preparation perfused through the vascular bed, and with glucose, 10 mM, present only in the vascular medium the addition of erythrocytes to the vascular infusate causes a significant reduction in both glucose utilization and in the rate of lactate production. The addition of erythrocytes to produce an haematocrit of 40% (v/v) causes a greater reduction in glucose utilization and lactate production than is found for an haematrocrit of 15%. About 10% of the lactate produced appears in the luminal contents. With an haematocrit of 15%, the O2 consumption of the whole wall of the jejunum was found to be 6-4 mumole O2 g dry wt.-1 min-1, equivalent of a value for the Q02 of 8-6 mul. O2 mg druwy wt.-1 hr-1. The uptake of O2 was almost entirely from the vascular infusate. 6...
机译：1.已在许多大鼠空肠非血管灌注制剂中测定了葡萄糖代谢为乳酸的速率。在一定条件下同时测量葡萄糖和乳酸的代谢，使得葡萄糖的摄取和乳酸的出现与时间成线性关系。 2.发现在厌氧或有氧培养的前45分钟内，大鼠空肠环利用葡萄糖的速率之间没有差异。在温育的前15分钟内，有氧条件下60-70％的代谢葡萄糖转化为乳酸。在接下来的30分钟内，该值增加到80-90％。在培养的0-15分钟期间，发现在厌氧条件下乳酸的产生高于在有氧条件下的乳酸产生，但是在此初始阶段之后，在有氧条件和厌氧条件下的乳酸产生速率是相同的。 3.对于通过在肠腔中循环营养液而在体外维持的大鼠空肠段，如果将制剂保持在无氧而非有氧条件下，则既不会刺激乳酸的产生速率，也不会刺激葡萄糖的利用。气体以气泡流的形式直接输送到肠腔（分段循环），减少了有氧条件下葡萄糖的利用和乳酸的产生。然而，在厌氧条件下未观察到对葡萄糖代谢的影响。用分段循环制剂而不是简单的再循环制剂发现巴斯德效应，与前一种制剂中有氧乳酸的产生速率较低有关。给出的理由是，在分段循环的条件下，可以更好地搅拌腔腔，从而增加O2进入组织的通道。 4.描述了通过血管床灌注的大鼠小肠的制剂。用这种制剂，葡萄糖的利用率显着低于再循环制剂的葡萄糖，并且乳酸盐的产生速率显着低于所研究的其他制剂。 5.在通过血管床灌注的制剂中，以及仅存在于血管介质中的10mM葡萄糖，向血管灌注液中添加红细胞会导致葡萄糖利用率和乳酸盐产生速率的显着降低。与产生15％的血细胞比容所发现的相比，添加红细胞以产生40％（v / v）的血细胞比容会导致更大的葡萄糖利用和乳酸产生的降低。产生的乳酸盐的约10％出现在腔内。在血细胞比容为15％的情况下，发现空肠整个壁的O2消耗量为6-4 mumole O2 g干wt.-1 min-1，相当于Q02值为8-6 mul。 O2 mg druwy wt.-1 hr-1。 O2的吸收几乎全部来自血管灌注液。 6 ...

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