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Zinc supplementation during in vitro maturation increases the production efficiency of cloned pigs

机译:体外成熟过程中补充锌可提高克隆猪的生产效率

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摘要

Zinc supplementation (0.8 µg/ml) in in vitro maturation (IVM) medium significantly enhances oocyte quality. In this study, we compared the development of somatic cell nuclear transfer (SCNT) embryos produced from conventional IVM (control) and zinc-supplemented IVM oocytes. A total of 1206 and 890 SCNT embryos were produced using control and zinc-supplemented oocytes, respectively, and then were transferred to 11 and 8 recipients, respectively. Five control recipients and three zinc-supplemented recipients became pregnant. Two live piglets and eight mummies were born from two control recipients, and ten live piglets and six stillborn piglets were born from three zinc-supplemented recipients. The production efficiency significantly increased in the zinc-supplemented group (0.33% vs. 3.02%). This report suggests that zinc supplementation in IVM medium improved the production efficiency of cloned pigs.
机译:在体外成熟(IVM)培养基中补充锌(0.8 µg / ml)可以显着提高卵母细胞质量。在这项研究中,我们比较了由常规IVM(对照)和补锌的IVM卵母细胞产生的体细胞核移植(SCNT)胚胎的发育。使用对照和补锌的卵母细胞分别产生了总共1206和890个SCNT胚胎,然后分别转移到11和8个接受者。五名对照接受者和三名补充锌的接受者怀孕。两名控制接受者出生了两只活猪和八只木乃伊,三名补充锌的接受者出生了十只活猪和六只死胎。补锌组的生产效率显着提高(0.33%对3.02%)。该报告表明,在IVM培养基中补充锌可提高克隆猪的生产效率。

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