首页> 美国卫生研究院文献>The Journal of Physiology >Slow calcium and potassium currents across frog muscle membrane: measurements with a vaseline-gap technique.
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Slow calcium and potassium currents across frog muscle membrane: measurements with a vaseline-gap technique.

机译:跨青蛙肌肉膜的钙和钾电流缓慢:使用凡士林间隙技术进行测量。

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摘要

1. A vaseline-gap voltage-clamp technique was used to record slow Ca2+ and K+ currents from frog skeletal muscle fibres loaded with the Ca2+ chelator EGTA. 2. K+ currents were increased when Mg2+ replaced external Ca2+, and they were abolished when internal K+ was replaced by tetraethylammonium (TEA+). Ca2+ currents could be studied in isolation in fibres loaded with (TEA)2EGTA. 3. Under maintained depolarization, Ca2+ currents slowly increase (half-time of 35 msec or more at 25 mV) and then decline to a steady value. Decline under repolarization is rapid (half-time of 6-7 msec) and complete. During an action potential, the Ca2+ influx through this system is probably less than the influx observed with tracers. 4. Ba2+, Sr2+, Ca2+, Mn2+ and Mg2+ can carry current across the membrane; Ni2+ and Co2+ cannot. Ca2+ currents are weakly blocked by external Mg2+.
机译:1.使用凡士林间隙电压钳技术记录负载有Ca2 +螯合剂EGTA的青蛙骨骼肌纤维中缓慢的Ca2 +和K +电流。 2.当Mg2 +替代外部Ca2 +时,K +电流增加,而当内部K +被四乙铵(TEA +)替代时,K +电流消失。可以在负载(TEA)2EGTA的光纤中隔离研究Ca2 +电流。 3.在维持去极化的情况下,Ca2 +电流缓慢增加(在25 mV时半衰期为35毫秒或更长),然后下降到稳定值。重新极化下的下降很快(半秒为6-7毫秒)且完全下降。在动作电位期间,通过该系统的Ca2 +流入量可能少于示踪剂所观察到的流入量。 4. Ba2 +,Sr2 +,Ca2 +,Mn2 +和Mg2 +可以使电流通过膜。 Ni2 +和Co2 +不能。 Ca2 +电流几乎不受外部Mg2 +的阻挡。

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