首页> 外文期刊>Brain research. Brain research protocols >Measurement of Ca(2+) currents in intact slow skeletal muscle fibers of the frog by the three-microelectrode technique.
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Measurement of Ca(2+) currents in intact slow skeletal muscle fibers of the frog by the three-microelectrode technique.

机译:通过三微电极技术测量青蛙完整的慢速骨骼肌纤维中的Ca(2+)电流。

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摘要

The recording of currents passing through calcium channels in intact skeletal muscle fibers presents several difficulties. However, use of the three-microelectrode voltage-clamp technique at the end of the fiber provided us with a good approximation of current values in such fibers. Using this technique, we were able to measure the calcium-channel current in slow skeletal muscle fibers of the frog (Rana pipiens) and to quantify the effects of denervation on this current.
机译:记录通过完整骨骼肌纤维中钙通道的电流存在一些困难。但是,在光纤末端使用三微电极电压钳技术为我们提供了此类光纤中电流值的良好近似值。使用该技术,我们能够测量青蛙(Rana pipiens)的慢速骨骼肌纤维中的钙通道电流,并量化去神经作用对该电流的影响。

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