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Expression of Vitreoscilla hemoglobin enhances production of arachidonic acid and lipids in Mortierella alpina

机译：玻璃藻血红蛋白的表达增强了高山被孢霉中花生四烯酸和脂质的产生

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摘要

BackgroundArachidonic acid (ARA, C20:4, n-6), which belongs to the omega-6 series of polyunsaturated fatty acids and has a variety of biological activities, is commercially produced in Mortierella alpina. Dissolved oxygen or oxygen utilization efficiency is a critical factor for Mortierella alpina growth and arachidonic acid production in large-scale fermentation. Overexpression of the Vitreoscilla hemoglobin gene is thought to significantly increase the oxygen utilization efficiency of the cells.

机译：背景技术花生四烯酸（ARA，C20：4，n-6）属于多不饱和脂肪酸的ω-6系列，并且具有多种生物学活性，其在高山被孢霉中商业化生产。溶解氧或氧气利用效率是大规模发酵中高山孢霉生长和花生四烯酸生产的关键因素。玻璃体血红蛋白基因的过表达被认为可以显着提高细胞的氧利用效率。

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期刊名称 BMC Biotechnology
作者
Huidan Zhang; Yingang Feng; Qiu Cui; Xiaojin Song;
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作者单位

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年(卷),期 2017(17),-1
年度 2017
页码 68
总页数 10
原文格式 PDF
正文语种
中图分类应用微生物学;
关键词
Arachidonic acid Mortierella Alpina Hemoglobin Dissolved oxygen Fermentation;

机译：花生四烯酸;高山铁耳;血红蛋白;溶解氧;发酵;

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专利

1. Expression of Vitreoscilla hemoglobin enhances production of arachidonic acid and lipids in Mortierella alpina [J] . Huidan Zhang, Yingang Feng, Qiu Cui, BMC Biotechnology . 2017,第1期

机译：玻璃藻血红蛋白的表达增强了高山被孢霉中花生四烯酸和脂质的产生
2. Production of 5,8,11-Eicosatrienoic Acid (Mead Acid) by a (Delta)6 Desaturation Activity-Enhanced Mutant Derived from a (Delta)12 Desaturase-Defective Mutant of an Arachidonic Acid-Producing Fungus, Mortierella alpina 1S-4. [J] . H Kawashima, M Nishihara, Y Hirano, Applied and Environmental Microbiology . 1997,第5期

机译：通过衍生自产花生四烯酸的真菌的Δδ12脱氢酶缺陷型突变体，高山被孢霉1S-4的Δ6去饱和活性增强的突变体，生产5,8,11-二十碳三烯酸（米德酸）。
3. Increased fatty acid unsaturation and production of arachidonic acid by homologous over-expression of the mitochondrial malic enzyme in Mortierella alpina [J] . Hao GF, Chen HQ, Du K, Biotechnology Letters . 2014,第9期

机译：同源过量表达线粒体苹果酸酶在高山被孢霉中增加了脂肪酸不饱和度和花生四烯酸的产生
4. Enhancement of Gellan Production in Sphingomonas paucimobilis JLJ by Heterogeneous Expression of Vitreoscilla Hemoglobin [C] . Qinglong Ji, Dan Li, Xiangqian Li, International conference on applied biotechnology . 2015

机译：玻璃体血球蛋白血红蛋白的异源表达增强了鞘氨醇单胞菌JLJ的结冷蛋白产量
5. Utilization of oil crop residues for enhanced algae based production of lipids, polyunsaturated fatty acids, and protein. [D] . Wang, Zhen. 2013

机译：利用油料作物残渣增强藻类生产脂质，多不饱和脂肪酸和蛋白质的能力。
6. Production of 5811-Eicosatrienoic Acid (Mead Acid) by a (Delta)6 Desaturation Activity-Enhanced Mutant Derived from a (Delta)12 Desaturase-Defective Mutant of an Arachidonic Acid-Producing Fungus Mortierella alpina 1S-4 [O] . H. Kawashima, M. Nishihara, Y. Hirano, 1997

机译：产自花生四烯酸真菌真菌阿尔门氏菌1S-4的Δ12脱氢酶缺陷型突变体衍生的Δ6去饱和活性增强型突变体的生产5811-二十碳三烯酸（米德酸）。
7. Increased fatty acid unsaturation and production of arachidonic acid by homologous over-expression of the mitochondrial malic enzyme in Mortierella alpina [O] . Guangfei Hao, Haiqin Chen, Kai Du, 2014

机译：通过Mistierella Alpina的线粒体苹果酶的同源过度表达增加脂肪酸不饱和度和制备arachidonic

Expression of Vitreoscilla hemoglobin enhances production of arachidonic acid and lipids in Mortierella alpina

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