• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>BMC Biotechnology >Expression of Vitreoscilla hemoglobin enhances production of arachidonic acid and lipids in Mortierella alpina
【24h】

Expression of Vitreoscilla hemoglobin enhances production of arachidonic acid and lipids in Mortierella alpina

机译：玻璃藻血红蛋白的表达增强了高山被孢霉中花生四烯酸和脂质的产生

获取原文
       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

BackgroundArachidonic acid (ARA, C20:4, n-6), which belongs to the omega-6 series of polyunsaturated fatty acids and has a variety of biological activities, is commercially produced in Mortierella alpina . Dissolved oxygen or oxygen utilization efficiency is a critical factor for Mortierella alpina growth and arachidonic acid production in large-scale fermentation. Overexpression of the Vitreoscilla hemoglobin gene is thought to significantly increase the oxygen utilization efficiency of the cells. ResultsAn optimized Vitreoscilla hemoglobin (VHb) gene was introduced into Mortierella alpina via Agrobacterium tumefaciens -mediated transformation. Compared with the parent strain, the VHb-expressing strain, termed VHb-20, grew faster under both limiting and non-limiting oxygen conditions and exhibited dramatic changes in cell morphology. Furthermore, VHb-20 produced 4- and 8-fold higher total lipid and ARA yields than those of the wild-type strain under a microaerobic environment. Furthermore, ARA production of VHb-20 was also 1.6-fold higher than that of the wild type under normal conditions. The results demonstrated that DO utilization was significantly increased by expressing the VHb gene in Mortierella alpina . ConclusionsThe expression of VHb enhances ARA and lipid production under both lower and normal dissolved oxygen conditions. This study provides a novel strategy and an engineered strain for the cost-efficient production of ARA.
机译：背景花生四烯酸（ARA，C20：4，n-6）属于omega-6系列多不饱和脂肪酸，具有多种生物学活性，是在高山被孢霉（Mertierella alpina）中商​​业生产的。溶解氧或氧气利用效率是大规模发酵中高山孢霉生长和花生四烯酸生产的关键因素。玻璃体血红蛋白基因的过表达被认为可以显着提高细胞的氧利用效率。结果通过根癌农杆菌介导的转化将优化的玻璃体血红蛋白（VHb）基因导入高山被孢霉。与亲本菌株相比，称为VHb-20的VHb表达菌株在限制性和非限制性氧条件下均生长更快，并且在细胞形态上表现出显着变化。此外，在有氧环境下，VHb-20的总脂质和ARA产量比野生型菌株高4到8倍。此外，在正常条件下，VHb-20的ARA产量也比野生型的高1.6倍。结果表明，通过在高山孢子菌中表达VHb基因可显着提高溶解氧利用率。结论在较低和正常溶解氧条件下，VHb的表达均可提高ARA和脂质的产生。这项研究为低成本生产ARA提供了一种新颖的策略和工程应变。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号