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Arachidonic acid production by Mortierella alpina with growth-coupled lipid synthesis

机译:高山被孢霉与生长耦合脂质合成的花生四烯酸生产

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The fungus Mortierella alpina LPM 301, a producer of arachidonic acid (ARA), was found to possess a unique property of a growth-coupled lipid sythesis. An increase in specific growth rate (u) from 0.03 to 0.05 h~(-1) resulted in a two-fold increase in the specific rate of lipid synthesis (milligram lipid (gram per lipid-free biomass) per hour. Under batch cultivation in glucose-containing media with urea or poetassium nitrate as nitrogen sources, the ARA content was 46.0 and 60.4/100 of lipid; 16.4 and 18.8/100 of dry biomass; and 4.2 and 4.5 g l~(-1), respectively. Under continuous cultivation of the strain, the productivity of ARA synthesis was 16.2 and 19.2 mg l~(-1) h~(-1) at u=0.05 and 0.03 h~(-1), respectively.
机译:发现了花生四烯酸(ARA)的生产者真菌高山被孢霉LPM 301,具有与生长耦合的脂质合成有关的独特特性。比生长速率(u)从0.03增加到0.05 h〜(-1),导致每小时脂质合成的比速率(毫克脂质(每无脂质生物质克数))增加两倍。在以尿素或硝酸钾为氮源的含葡萄糖培养基中,ARA含量分别为脂质的46.0和60.4 / 100,干燥生物质的16.4和18.8 / 100,以及4.2和4.5 gl〜(-1)。培养菌株,在u = 0.05和0.03 h〜(-1)时,ARA合成的生产力分别为16.2 mg l〜(-1)h〜(-1)。

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