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Correlation analysis of two-dimensional gel electrophoretic protein patterns and biological variables

机译:二维凝胶电泳蛋白质图谱与生物学变量的相关性分析

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摘要

BackgroundTwo-dimensional gel electrophoresis (2DE) is a powerful technique to examine post-translational modifications of complexly modulated proteins. Currently, spot detection is a necessary step to assess relations between spots and biological variables. This often proves time consuming and difficult when working with non-perfect gels. We developed an analysis technique to measure correlation between 2DE images and biological variables on a pixel by pixel basis. After image alignment and normalization, the biological parameters and pixel values are replaced by their specific rank. These rank adjusted images and parameters are then put into a standard linear Pearson correlation and further tested for significance and variance.
机译:背景技术二维凝胶电泳(2DE)是一种检查复杂调节蛋白的翻译后修饰的强大技术。当前,斑点检测是评估斑点与生物学变量之间关系的必要步骤。当使用不完美的凝胶时,这通常被证明是耗时且困难的。我们开发了一种分析技术,可以逐个像素地测量2DE图像和生物学变量之间的相关性。在图像对齐和归一化之后,生物学参数和像素值将替换为其特定等级。然后将这些经过等级调整的图像和参数放入标准线性Pearson相关中,并进一步测试显着性和方差。

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