TWO-DIMENSIONAL ELECTROPHORETIC ANALYSIS OF HUMAN MILK-FAT-GLOBULE MEMBRANE PROTEINS WITH ATTENTION TO APOLIPOPROTEIN E PATTERNS

Goldfarb M.

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TWO-DIMENSIONAL ELECTROPHORETIC ANALYSIS OF HUMAN MILK-FAT-GLOBULE MEMBRANE PROTEINS WITH ATTENTION TO APOLIPOPROTEIN E PATTERNS

机译：着眼于载脂蛋白E模式的人乳-脂肪-球状膜蛋白的二维电泳分析

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Two-dimensional (2-D; Iso Dalt method) electrophoresis was done on lipid membrane fractions from human milk samples collected in polypropylene tubes at home by the mother. The samples represent milk from two weeks to ten months post partum. Identification was made by immunoassay of Western blots. Apolipoprotein E was not easily seen on silver-stained gels because concentration is small and spots overlap another protein. Blot assays show a more complex pattern than 34 kDa plasma apo E with molecular size isoforms at 36 kDa, 34 kDa, 20 kDa and 15 kDa. One sample was negative for apo E. Apo E plays an important role in lipid transport in tissue and in the brain. It is synthesized in a variety of tissues by specific cells. Milk may reflect several sources. [References: 26]

机译：母亲在家中从聚丙烯管中收集的人乳样品中的脂膜部分进行了二维（2-D; Iso Dalt方法）电泳。样品代表产后两周到十个月的牛奶。通过Western印迹的免疫测定进行鉴定。在载银染色的凝胶上不容易看到载脂蛋白E，因为浓度很小且斑点与另一种蛋白重叠。印迹分析显示比34 kDa血浆载脂蛋白E更复杂的模式，其分子大小同工型分别为36 kDa，34 kDa，20 kDa和15 kDa。一个样品对apo E阴性。ApoE在组织和大脑中的脂质转运中起重要作用。它由特定细胞在多种组织中合成。牛奶可能反映了几种来源。 [参考：26]

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《Electrophoresis: The Official Journal of the International Electrophoresis Society》 |1997年第4期|共5页
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Goldfarb M.;
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正文语种 eng
中图分类化学;
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Two-dimensional polyacrylamide gel electrophoresis; Milk; Apolipoprotein e; Bovine butyrophilin; Mammary tissue; Glycoprotein; Binding; Gels; Beta-2-glycoprotein-i; Antibodies; Complex;

机译：二维聚丙烯酰胺凝胶电泳;牛奶;载脂蛋白e;牛酪氨酸蛋白;乳腺组织;糖蛋白;结合;凝胶;β2-糖蛋白-i;抗体;复合物;

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1. TWO-DIMENSIONAL ELECTROPHORETIC ANALYSIS OF HUMAN MILK-FAT-GLOBULE MEMBRANE PROTEINS WITH ATTENTION TO APOLIPOPROTEIN E PATTERNS [J] . Goldfarb M. Electrophoresis: The Official Journal of the International Electrophoresis Society . 1997,第3a4期

机译：着眼于载脂蛋白E模式的人乳-脂肪-球状膜蛋白的二维电泳分析
2. A two-dimensional electrophoretic analysis of the proteins and glycoproteins of liver plasma membrane domains and endosomes. Implications for endocytosis and transcytosis [J] . C Enrich, P Tabona, W H Evans The biochemical journal . 1990,第1期

机译：肝质膜结构域和内体的蛋白质和糖蛋白的二维电泳分析。对内吞和转胞吞的影响
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机译：人类载脂蛋白CIII（apoCIII）基因和人类载脂蛋白E（apoE）蛋白的研究。
6. Correlation analysis of two-dimensional gel electrophoretic protein patterns and biological variables [O] . Werner Van Belle, Nina Ånensen, Ingvild Haaland, 2006

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TWO-DIMENSIONAL ELECTROPHORETIC ANALYSIS OF HUMAN MILK-FAT-GLOBULE MEMBRANE PROTEINS WITH ATTENTION TO APOLIPOPROTEIN E PATTERNS

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