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首页> 美国卫生研究院文献>Biochemical Journal >Effects of starvation diabetes and acute insulin treatment on the regulation of polypeptide-chain initiation in rat skeletal muscle.
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Effects of starvation diabetes and acute insulin treatment on the regulation of polypeptide-chain initiation in rat skeletal muscle.

机译：饥饿糖尿病和急性胰岛素治疗对大鼠骨骼肌多肽链起始调控的影响。

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The rate of protein synthesis in skeletal muscle is greatly decreased in response to diabetes and starvation. Analysis of polyribosome profiles indicates that polypeptide-chain initiation is impaired under these conditions. To identify the step in initiation that is affected, we assayed the incorporation of [35S]methionyl-tRNAfMet into [35S]methionyl-tRNAfMet . 40S-ribosomal-subunit initiation complexes in cell-free extracts based on postmitochondrial supernatants prepared from gastrocnemius muscle. Extracts from either starved or diabetic rats were 30-40% less active in forming these complexes compared with those derived from fed or insulin-maintained controls respectively. This change could be reversed by treatment of either starved or diabetic rats with insulin in vivo 30 min before death. Formation of 40S initiation complexes by extracts from either fed or starved rats could be stimulated by the addition of exogenous purified initiation factor eIF-2, but extracts from starved or diabetic rats were more sensitive than controls to stimulation by low concentrations of the factor. These results provide evidence for the acute regulation by insulin of protein synthesis in skeletal muscle at the level of polypeptide-chain initiation, and suggest that in this tissue, as in certain other eukaryotic systems, control of initiation appears to be mediated by changes in the activity of initiation factor eIF-2.
机译：响应于糖尿病和饥饿，骨骼肌中蛋白质合成的速率大大降低。多核糖体谱分析表明，在这些条件下多肽链的启动受到了损害。为了确定引发的起始步骤，我们分析了将[35S]甲硫酰基-tRNAfMet掺入[35S]甲硫酰基-tRNAfMet中的方法。基于从腓肠肌制备的线粒体后上清液的无细胞提取物中的40S-核糖体亚基起始复合物。与分别来自进食或胰岛素维持的对照组的提取物相比，饥饿或糖尿病大鼠的提取物形成这些复合物的活性低30-40％。这种变化可以通过在死前30分钟在体内用胰岛素治疗饥饿或糖尿病的大鼠来逆转。可以通过添加外源的纯化起始因子eIF-2来刺激从进食或饥饿的大鼠提取物形成40S起始复合物，但是饥饿或糖尿病大鼠的提取物对对照的敏感性比低浓度因子低。这些结果提供了胰岛素在多肽链起始水平上对骨骼肌中蛋白质合成的急性调节的证据，并表明在该组织中，如在某些其他真核系统中一样，起始控制似乎是由胰岛素的变化介导的。起始因子eIF-2的活性。

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