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首页> 美国卫生研究院文献>Biochemical Journal >Maximum activities of key enzymes of glycolysis glutaminolysis pentose phosphate pathway and tricarboxylic acid cycle in normal neoplastic and suppressed cells.
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Maximum activities of key enzymes of glycolysis glutaminolysis pentose phosphate pathway and tricarboxylic acid cycle in normal neoplastic and suppressed cells.

机译：在正常赘生性和抑制性细胞中糖酵解谷氨酰胺分解磷酸戊糖途径和三羧酸循环的关键酶的最大活性。

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摘要

1. Maximal activities of some key enzymes of glycolysis, the pentose phosphate pathway, the tricarboxylic acid cycle and glutaminolysis were measured in homogenates from a variety of normal, neoplastic and suppressed cells. 2. The relative activities of hexokinase and 6-phosphofructokinase suggest that, particularly in neoplastic cells, in which the capacity for glucose transport is high, hexokinase could approach saturation in respect to intracellular glucose; consequently, hexokinase and phosphofructokinase could play an important role in the regulation of glycolytic flux in these cells. 3. The activity of pyruvate kinase is considerably higher in tumorigenic cells than in non-tumorigenic cells and higher in metastatic cells than in tumorigenic cells: for non-tumorigenic cells the activities range from 28.4 to 574, for tumorigenic cells from 899 to 1280, and for metastatic cells from 1590 to 1627 nmol/min per mg of protein. 4. The ratio of pyruvate kinase activity to 2 x phosphofructokinase activity is very high in neoplastic cells. The mean is 22.4 for neoplastic cells, whereas for muscle from 60 different animals it is only 3.8. 5. Both citrate synthase and isocitrate dehydrogenase activities are present in non-neoplastic and neoplastic cells, suggesting that the full complement of tricarboxylic-acid-cycle enzymes are present in these latter cells. 6. In neoplastic cells, the activity of glutaminase is similar to or greater than that of hexokinase, which suggests that glutamine may be as important as glucose for energy generation in these cells.
机译：1.在来自各种正常，肿瘤和抑制细胞的匀浆中，测量了糖酵解，磷酸戊糖途径，三羧酸循环和谷氨酰胺分解的一些关键酶的最大活性。 2.己糖激酶和6-磷酸果糖激酶的相对活性表明，特别是在葡萄糖转运能力高的肿瘤细胞中，己糖激酶相对于细胞内葡萄糖可能接近饱和。因此，己糖激酶和磷酸果糖激酶可能在这些细胞的糖酵解通量调节中起重要作用。 3.丙酮酸激酶在致瘤细胞中的活性明显高于非致瘤细胞，在转移细胞中的活性高于致瘤细胞：对于非致瘤细胞，其活性范围为28.4至574，对于致癌细胞为899至1280，对于转移细胞，每毫克蛋白质的释放速率为1590至1627 nmol / min。 4.在肿瘤细胞中丙酮酸激酶活性与2×磷酸果糖激酶活性之比非常高。肿瘤细胞的平均值为22.4，而来自60种不同动物的肌肉的平均值仅为3.8。 5.柠檬酸合酶和异柠檬酸脱氢酶的活性都存在于非肿瘤和肿瘤细胞中，这表明三羧酸循环酶的完全互补存在于这些细胞中。 6.在赘生性细胞中，谷氨酰胺酶的活性类似于或大于己糖激酶的活性，这表明谷氨酰胺对于这些细胞中的能量产生可能与葡萄糖一样重要。

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