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首页> 美国卫生研究院文献>Balkan Journal of Medical Genetics : BJMG >Novel Patterns of the Epstein-Barr Nuclear Antigen (EBNA-1) V-Val Subtype in EBV-associated Nasopharyngeal Carcinoma from Vietnam
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Novel Patterns of the Epstein-Barr Nuclear Antigen (EBNA-1) V-Val Subtype in EBV-associated Nasopharyngeal Carcinoma from Vietnam

机译：来自越南的EBV相关鼻咽癌中的爱泼斯坦-巴尔核抗原（EBNA-1）V-Val亚型的新型模式。

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摘要

The Epstein-Barr nuclear antigen 1 (EBNA-1) gene, plays a key role in viral infection, immortalization, viral genome replication, transcription and maintenance, and is the frequently detected gene, protein in both latent and lytic stage of Epstein-Barr virus (EBV). Based on the amino acid at position 487, EBNA-1 was classified into five subtypes, including P-Ala, P-Thr, V-Val, V-Pro and V-Leu. In Vietnam, an Asian country with a high incidence, mortality rates of nasopharyngeal carcinoma (NPC), had limited research on the EBNA-1 variation. Therefore, the aim of the current study was to identify the pattern of the EBNA-1 V-Val subtype in Vietnamese NPC patients, for its value further applied in NPC patients. Fifty-eight NPC biopsy samples were collected from local patients, analyzed by nested-polymerase chain reaction (nested-PCR), sequencing and compared to a previous B95-8 prototype sequence. Four EBNA-1 subtypes, including V-Val (35/44, 79.55%), P-Ala (2/44, 4.55%), P-Thr (5/44, 11.36%), and V-Leu (2/44, 4.55%), were observed in 44/58 samples. The sequences of the V-Val subtype were compared to the B95-8 prototype, resulting in five patterns, contained seven consensus changes, including five amino acid changes at positions 487, 499, 502, 524, 594, and two silent changes at residues 520 and 553. Of these, four of five, patterns were identified as novel patterns of the V-Val subtype, showing the different changes of amino acids at positions 492, 528, 529, 553, 585 and 588, by comparison with previous studies of V-Val EBNA-1. Those data suggested the profile of variation patterns of the EBNA-1 gene, related to geographic distribution, in Vietnamese NPC patients.
机译：Epstein-Barr核抗原1（EBNA-1）基因在病毒感染，永生化，病毒基因组复制，转录和维持中起关键作用，是Epstein-Barr潜伏期和裂解期经常检测到的基因，蛋白质病毒（EBV）。基于487位氨基酸，EBNA-1被分为五种亚型，包括P-Ala，P-Thr，V-Val，V-Pro和V-Leu。在越南这个亚洲国家，鼻咽癌（NPC）的死亡率很高，因此对EBNA-1变异的研究有限。因此，本研究的目的是确定越南NPC患者中EBNA-1 V-Val亚型的模式，为其在NPC患者中的进一步应用提供价值。从本地患者中收集了58份NPC活检样本，通过巢式聚合酶链反应（巢式PCR）进行分析，测序，并与以前的B95-8原型序列进行了比较。四种EBNA-1亚型，包括V-Val（35/44，79.55％），P-Ala（2/44，4.55％），P-Thr（5/44，11.36％）和V-Leu（2 /在44/58个样本中观察到44个（4.55％）。将V-Val亚型的序列与B95-8原型进行比较，得出五种模式，包含七个共有变化，包括在487、499、502、524、594位的五个氨基酸变化，以及两个残基的沉默变化520和553。在五种模式中，有四种被确定为V-Val亚型的新型模式，与以前的研究相比，显示了492、528、529、553、585和588位氨基酸的不同变化V-Val EBNA-1。这些数据表明越南NPC患者中EBNA-1基因的变异模式与地理分布有关。

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