• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Balkan journal of medical genetics: BJMG >Novel patterns of the Epstein-Barr nuclear antigen (EBNA-1) V-Val subtype in EBV-associated nasopharyngeal carcinoma from Vietnam
【24h】

Novel patterns of the Epstein-Barr nuclear antigen (EBNA-1) V-Val subtype in EBV-associated nasopharyngeal carcinoma from Vietnam

机译：来自越南的EBV相关的鼻咽癌中EPStein-BART核抗原（EBNA-1）V-VAL亚型的新型模式

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The Epstein-Barr nuclear antigen 1 (EBNA-1) gene, plays a key role in viral infection, immortalization, viral genome replication, transcription and maintenance, and is the frequently detected gene, protein in both latent and lytic stage of Epstein-Barr virus (EBV). Based on the amino acid at position 487, EBNA-1 was classified into five subtypes, including P-Ala, P-Thr, V-Val, V-Pro and V-Leu. In Vietnam, an Asian country with a high incidence, mortality rates of nasopharyngeal carcinoma (NPC), had limited research on the EBNA-1 variation. Therefore, the aim of the current study was to identify the pattern of the EBNA-1 V-Val subtype in Vietnamese NPC patients, for its value further applied in NPC patients. Fifty-eight NPC biopsy samples were collected from local patients, analyzed by nested-polymerase chain reaction (nested-PCR), sequencing and compared to a previous B95-8 prototype sequence. Four EBNA-1 subtypes, including V-Val (35/44, 79.55%), P-Ala (2/44, 4.55%), P-Thr (5/44, 11.36%), and V-Leu (2/44, 4.55%), were observed in 44/58 samples. The sequences of the V-Val subtype were compared to the B95-8 prototype, resulting in five patterns, contained seven consensus changes, including five amino acid changes at positions 487, 499, 502, 524, 594, and two silent changes at residues 520 and 553. Of these, four of five, patterns were identified as novel patterns of the V-Val subtype, showing the different changes of amino acids at positions 492, 528, 529, 553, 585 and 588, by comparison with previous studies of V-Val EBNA-1. Those data suggested the profile of variation patterns of the EBNA-1 gene, related to geographic distribution, in Vietnamese NPC patients.
机译：Epstein-Barr核抗原1（EBNA-1）基因在病毒感染，永生化，病毒基因组复制，转录和维护中起着关键作用，并且是Epstein-Barr的潜在和抒情阶段的经常检测到的基因，蛋白质病毒（EBV）。基于位于487的氨基酸，EBNA-1分为五个亚型，包括P-ALA，P-THR，V-VAL，V-Pro和V-Leu。在越南，一个具有高发病率的亚洲国家，鼻咽癌癌（NPC）的死亡率，对EBNA-1变异有限。因此，目前研究的目的是鉴定越南NPC患者的EBNA-1V-VAL亚型的模式，其价值进一步应用于NPC患者。从局部患者中收集58个NPC活检样品，通过巢式聚合酶链反应（嵌套-PCR）分析，测序并与先前的B95-8原型序列进行测序。四个EBNA-1亚型，包括V-VAL（35/44,79.55％），P-ALA（2/44,4.55％），P-THR（5/44,11.36％）和V-Leu（2 / 44,4.55％）在44/58样品中观察到。将V-Val亚型的序列与B95-8原型进行比较，导致五种模式，含有七种共识变化，包括在487,499,502,524,594的位置的五个氨基酸变化和残留物的两个静音变化其中50和553.其中五种，其中四种模式被鉴定为V-Val亚型的新型模式，显示通过与先前研究相比，氨基酸在第492,528,529,553,585和588处的不同变化V-Val EBNA-1。这些数据建议在越南NPC患者中建议EBNA-1基因的变异模式的概况与地理分布相关。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号