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首页> 美国卫生研究院文献>Applied and Environmental Microbiology >Comparison of methods for recovery of Escherichia coli and Staphylococcus aureus from seeded laundry fabrics.
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Comparison of methods for recovery of Escherichia coli and Staphylococcus aureus from seeded laundry fabrics.

机译：比较从种子洗衣织物中回收大肠杆菌和金黄色葡萄球菌的方法。

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To assess the effect of laundry procedures on fabric-associated bacteria, a standard method of enumeration is needed. We evaluated six methods for enumeration of Escherichia coli and Staphylococcus aureus seeded (10(2) and 10(5) CFU/100 cm2 of fabric area) onto sterilized hospital sheets and terry . Two methods involved maceration of seeded swatches in broth followed by passage of the broth through a 0.45-micron-pore-size, 47-mm-diameter filter membrane. Three methods involved agitation of seeded swatches in broth with a paint shaker and membrane filtration of the broth to recover eluted bacterial cells, and the final method involved direct enumeration of cells on fabrics by overlaying seeded swatches with agar containing triphenyltetrazolium chloride as an indicator. The most convenient recovery method employed a 90-s agitation followed by serial dilution of broths and membrane filtration. This method provided 44/57% (low seed/high seed) recovery of E. coli from sheets and 133/31% from terry and 34/74% recovery of S. aureus from sheets and 58/57% from terry . Although maceration provided similar recovery of E. coli and S. aureus, it is a less-practical method. The direct enumeration method was ineffective for enumerating gram-positive bacteria. We conclude that either the agitation or maceration method used enumerated the seeded bacteria to within 1 log10 of their expected number and can be used to assess the bactericidal effectiveness of various steps in the laundering process.
机译：为了评估洗衣程序对织物相关细菌的影响，需要一种标准的枚举方法。我们评估了六种方法的计数方法，即将播种的大肠杆菌和金黄色葡萄球菌（织物面积10（2）和10（5）CFU / 100 cm2）枚举到无菌的医院床单和毛圈上。两种方法包括浸入肉汤样在肉汤中，然后使肉汤通过0.45微米孔径，直径47毫米的滤膜。三种方法包括用油漆搅拌器在肉汤中搅拌种子样板，并用肉汤进行膜过滤以回收洗脱的细菌细胞，最后一种方法是通过在种子样板上覆盖含氯化三苯四唑的琼脂来直接枚举织物上的细胞。最方便的回收方法是90s搅拌，然后连续稀释肉汤并进行膜过滤。该方法从薄片中回收了44.57％（低种子/高种子）的大肠杆菌，从毛圈中回收了133/31％，从薄片中回收了金黄色葡萄球菌，从毛圈中回收了58/57％。尽管浸软提供了类似的大肠杆菌和金黄色葡萄球菌的恢复，但它是一种不太实用的方法。直接枚举方法无法枚举革兰氏阳性细菌。我们得出的结论是，所使用的搅拌法或浸渍法可将种子细菌枚举到其预期数量的1 log10以内，并可用于评估洗衣过程中各个步骤的杀菌效果。

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