首页> 美国卫生研究院文献>Applied and Environmental Microbiology >Genetic Rearrangement of Plasmids: In Vivo Recombination between a Dehalogenation Plasmid and Multiple-Resistance Plasmid RP4 in Pseudomonas sp
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Genetic Rearrangement of Plasmids: In Vivo Recombination between a Dehalogenation Plasmid and Multiple-Resistance Plasmid RP4 in Pseudomonas sp

机译:质粒的遗传重排:假单胞菌属种中的脱卤素质粒和多重耐药质粒RP4之间的体内重组。

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摘要

When Moraxella plasmid pUO1 encoding haloacetate dehalogenase and mercury resistance coexisted with IncP-1 plasmid RP4 in Pseudomonas sp., genetic exchange between the plasmids often occurred, probably by site-specific recombination. The recombinant plasmids obtained were classified into four groups on the basis of phenotype. Representative plasmids for each group were analyzed for DNA composition and function, and the mechanism for the formation of these plasmids was sought. They were inherited stably in Escherichia coli and a Pseudomonas sp.
机译:当假单胞菌属物种中编码卤代乙酸盐脱卤素酶和耐汞性的莫拉氏菌质粒pUO1与IncP-1质粒RP4共存时,质粒之间经常发生基因交换,可能是通过位点特异性重组。根据表型将获得的重组质粒分为四类。分析每组的代表性质粒的DNA组成和功能,并寻找形成这些质粒的机制。它们在大肠杆菌和假单胞菌属种中稳定遗传。

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