• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Applied and Environmental Microbiology >A Novel Regulatory Gene Tri10 Controls Trichothecene Toxin Production and Gene Expression
【2h】

A Novel Regulatory Gene Tri10 Controls Trichothecene Toxin Production and Gene Expression

机译：新型调节基因Tri10控制天花粉毒素的产生和基因表达

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We report here the characterization of Tri10, a novel regulatory gene within the trichothecene gene cluster. Comparison of Tri10 genomic and mRNA sequences revealed that removal of a single 77-bp intron provided a 1,260-bp open reading frame, encoding a 420-amino-acid protein. Disruption of Tri10 in Fusarium sporotrichioides abolished T-2 toxin production and dramatically decreased the transcript accumulation for four trichothecene genes (Tri4, Tri5, Tri6, and Tri101) and an apparent farnesyl pyrophosphate synthetase (Fpps) gene. Conversely, homologous integration of a disruption vector by a single upstream crossover event significantly increased T-2 toxin production and elevated the transcript accumulation of the trichothecene genes and Fpps. Further analysis revealed that disruption of Tri10, and to a greater extent the disruption of Tri6, increased sensitivity to T-2 toxin under certain growth conditions. Although Tri10 is conserved in Fusarium graminearum and Fusarium sambucinum and clearly plays a central role in regulating trichothecene gene expression, it does not show any significant matches to proteins of known or predicted function or to motifs except a single transmembrane domain. We suggest a model in which Tri10 acts upstream of the cluster-encoded transcription factor TRI6 and is necessary for full expression of both the other trichothecene genes and the genes for the primary metabolic pathway that precedes the trichothecene biosynthetic pathway, as well as for wild-type levels of trichothecene self-protection. We further suggest the presence of a regulatory loop where Tri6 is not required for the transcription of Tri10 but is required to limit the expression of Tri10.
机译：我们在此报告Tri10的特征，Tri10是单端孢菌素基因簇中的新型调控基因。 Tri10基因组和mRNA序列的比较显示，去除单个77 bp的内含子可提供一个1,260 bp的开放阅读框，编码420个氨基酸的蛋白质。镰孢镰刀菌中Tri10的破坏消除了T-2毒素的产生，并显着降低了四个四茂铁基因（Tri4，Tri5，Tri6和Tri101）和一个明显的法呢基焦磷酸合成酶（Fpps）基因的转录物积累。相反，单个上游交换事件对破坏载体的同源整合显着增加了T-2毒素的产生并提高了单端孢菌素基因和Fpps的转录本积累。进一步的分析表明，在某些生长条件下，Tri10的破坏以及更大程度上Tri6的破坏增加了对T-2毒素的敏感性。尽管Tri10在禾谷镰刀菌和镰刀镰刀菌中是保守的，并且显然在调节单端孢菌素基因表达中起着核心作用，但除单个跨膜结构域外，它与已知或预测功能的蛋白质或基序没有任何显着匹配。我们建议建立一个模型，其中Tri10在簇编码的转录因子TRI6的上游起作用，并且对于其他天花粉基因和天花粉生物合成途径之前的主要代谢途径以及野生动物的全基因表达都是必需的单端孢菌素自我保护的类型水平。我们进一步建议存在一个调节环，其中 Tri10 的转录不是必需的，而 Tri6 却是限制 Tri10的表达所必需的。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号