• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Applied and Environmental Microbiology >Comparison of Microbial Community Compositions of Two Subglacial Environments Reveals a Possible Role for Microbes in Chemical Weathering Processes
【2h】

Comparison of Microbial Community Compositions of Two Subglacial Environments Reveals a Possible Role for Microbes in Chemical Weathering Processes

机译：比较两个冰川以下环境中的微生物群落组成揭示了微生物在化学风化过程中的可能作用

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Viable microbes have been detected beneath several geographically distant glaciers underlain by different lithologies, but comparisons of their microbial communities have not previously been made. This study compared the microbial community compositions of samples from two glaciers overlying differing bedrock. Bulk meltwater chemistry indicates that sulfide oxidation and carbonate dissolution account for 90% of the solute flux from Bench Glacier, Alaska, whereas gypsum/anhydrite and carbonate dissolution accounts for the majority of the flux from John Evans Glacier, Ellesmere Island, Nunavut, Canada. The microbial communities were examined using two techniques: clone libraries and dot blot hybridization of 16S rRNA genes. Two hundred twenty-seven clones containing amplified 16S rRNA genes were prepared from subglacial samples, and the gene sequences were analyzed phylogenetically. Although some phylogenetic groups, including the Betaproteobacteria, were abundant in clone libraries from both glaciers, other well-represented groups were found at only one glacier. Group-specific oligonucleotide probes were developed for two phylogenetic clusters that were of particular interest because of their abundance or inferred biochemical capabilities. These probes were used in quantitative dot blot hybridization assays with a range of samples from the two glaciers. In addition to shared phyla at both glaciers, each glacier also harbored a subglacial microbial population that correlated with the observed aqueous geochemistry. These results are consistent with the hypothesis that microbial activity is an important contributor to the solute flux from glaciers.
机译：在不同的岩性下，在几个地理上远的冰川下已经发现了活的微生物，但是以前没有对它们的微生物群落进行比较。这项研究比较了覆盖在不同基岩上的两个冰川样品的微生物群落组成。大量的融水化学表明，硫化物的氧化和碳酸盐的溶解占阿拉斯加Bench Glacier溶质通量的90％，而石膏/硬石膏和碳酸盐的溶解占加拿大努纳武特Ellesmere Island John Evans冰川的大部分通量。使用两种技术检查微生物群落：克隆文库和16S rRNA基因的斑点印迹杂交。从冰下样品中制备了包含扩增的16S rRNA基因的277个克隆，并对这些基因序列进行了系统发育分析。尽管在两个冰川的克隆文库中都有一些系统发育类群，包括Betaproteobacteria，但在一个冰川中却发现了其他代表性良好的类群。针对两个系统发生簇开发了群体特异性寡核苷酸探针，由于它们的丰度或推断的生化能力，它们引起了人们的特别关注。这些探针与来自两个冰川的一系列样品一起用于定量斑点印迹杂交测定。除了两个冰川都有共同的门外，每个冰川还包含与观测到的含水地球化学相关的冰川下微生物种群。这些结果与微生物活动是冰川溶质通量的重要贡献者这一假设相吻合。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号