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Simple Adhesive-Tape-Based Sampling of Tomato Surfaces Combined with Rapid Fluorescence In Situ Hybridization for Salmonella Detection

机译：简单的基于胶带的番茄表面采样结合快速荧光原位杂交技术检测沙门氏菌

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摘要

A simple adhesive-tape-based method for sampling of tomato surfaces was combined with fluorescence in situ hybridization for rapid culture-independent detection of Salmonella strains. Tapes could also be placed face-down on selective agar for on-tape enrichment of captured Salmonella cells. Overlay of cell-charged tapes with small volumes of liquid enrichment media enabled subsequent detection of tape-captured Salmonella via flow cytometry.

机译：一种简单的基于胶带的番茄表面采样方法与荧光原位杂交相结合，可快速独立于培养物检测沙门氏菌菌株。也可以将胶带正面朝下放置在选择性琼脂上，以在条带上富集捕获的沙门氏菌细胞。用少量液体富集培养基覆盖的带电胶带可以通过流式细胞仪随后检测被胶带捕获的沙门氏菌。

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期刊名称 Applied and Environmental Microbiology
作者
Bledar Bisha; Byron F. Brehm-Stecher;
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作者单位

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年(卷),期 2009(75),5
年度 2009
页码 1450–1455
总页数 6
原文格式 PDF
正文语种
中图分类应用微生物学;微生物学;
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专利

1. Combined capillary electrophoresis and DNA-fluorescence in situ hybridization for rapid molecular identification of Salmonella Typhimurium in mixed culture. [J] . Lantz AW, Brehm-Stecher BF, Armstrong DW Electrophoresis: The Official Journal of the International Electrophoresis Society . 2008,第12期

机译：毛细管电泳和DNA荧光原位杂交相结合，可快速鉴定混合培养中鼠伤寒沙门氏菌的分子。
2. Rapid Quantitative Detection of Salmonella enterica Using Fluorescence In Situ Hybridization with Filter-cultivation (FISHFC) Method [J] . Shigemasa Shimizu, Ryouhei Aoi, Yui Osanai, Food science and technology research . 2013,第1期

机译：荧光原位杂交-滤培养法快速定量检测肠炎沙门氏菌
3. A simple and rapid method for combining fluorescent in situ RNA hybridization (FISH) and immunofluorescence in the C. elegans germline [J] . Dong Suk Yoon, DeQwon L. Pendergrass, Myon-Hee Lee MethodsX . 2016,第1期

机译：一种简单快速的方法，可以在 C中结合荧光原位RNA杂交（FISH）和免疫荧光。线虫种系
4. Novel, rapid DNA-based on-chip bacterial identification system combining dielectrophoresis and amplification-free fluorescent resonance energy transfer assisted in-situ hybridization (FRET-ISH) [C] . Michelle M. Packard, Maxim Shusteff, Evangelyn C. Alocilja Biosensing and nanomedicine IV . 2011

机译：基于介电电泳和无扩增荧光共振能量转移辅助原位杂交的新型快速，基于DNA的片上细菌鉴定系统（FRET-ISH）
5. Fluorescence in situ hybridization-based detection of Salmonella spp. and Listeria monocytogenes in complex food matrices. [D] . Bisha, Bledar. 2009

机译：基于荧光原位杂交的沙门氏菌检测。和复杂食品基质中的单核细胞增生李斯特菌。
6. Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce [O] . Bledar Bisha, Byron F. Brehm-Stecher 2010

机译：胶粘带采样与荧光原位杂交相结合可快速检测新鲜农产品上的沙门氏菌
7. Simple Adhesive-Tape-Based Sampling of Tomato Surfaces Combined with Rapid Fluorescence In Situ Hybridization for Salmonella Detection [O] . Bisha Bledar, Brehm-Stecher Byron F. 2009

机译：简单的基于胶带的番茄表面采样结合快速荧光原位杂交技术检测沙门氏菌

Simple Adhesive-Tape-Based Sampling of Tomato Surfaces Combined with Rapid Fluorescence In Situ Hybridization for Salmonella Detection

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