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首页> 美国卫生研究院文献>Applied and Environmental Microbiology >Syntrophs Dominate Sequences Associated with the Mercury Methylation-Related Gene hgcA in the Water Conservation Areas of the Florida Everglades
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Syntrophs Dominate Sequences Associated with the Mercury Methylation-Related Gene hgcA in the Water Conservation Areas of the Florida Everglades

机译：在佛罗里达大沼泽地的水源保护区与细菌甲基化相关的基因hgcA相关的突触体占主导地位。

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The mechanisms and rates of mercury methylation in the Florida Everglades are of great concern because of potential adverse impacts on human and wildlife health through mercury accumulation in aquatic food webs. We developed a new PCR primer set targeting hgcA, a gene encoding a corrinoid protein essential for Hg methylation across broad phylogenetic boundaries, and used this primer set to study the distribution of hgcA sequences in soils collected from three sites along a gradient in sulfate and nutrient concentrations in the northern Everglades. The sequences obtained were distributed in diverse phyla, including Proteobacteria, Chloroflexi, Firmicutes, and Methanomicrobia; however, hgcA clone libraries from all sites were dominated by sequences clustering within the order Syntrophobacterales of the Deltaproteobacteria (49 to 65% of total sequences). dsrB mRNA sequences, representing active sulfate-reducing prokaryotes at the time of sampling, obtained from these sites were also dominated by Syntrophobacterales (75 to 89%). Laboratory incubations with soils taken from the site low in sulfate concentrations also suggested that Hg methylation activities were primarily mediated by members of the order Syntrophobacterales, with some contribution by methanogens, Chloroflexi, iron-reducing Geobacter, and non-sulfate-reducing Firmicutes inhabiting the sites. This suggests that prokaryotes distributed within clades defined by syntrophs are the predominant group controlling methylation of Hg in low-sulfate areas of the Everglades. Any strategy for managing mercury methylation in the Everglades should consider that net mercury methylation is not limited to the action of sulfate reduction.
机译：佛罗里达大沼泽地地区汞甲基化的机理和速率引起了极大的关注，因为水生食物网中的汞积累可能对人类和野生动植物健康产生不利影响。我们开发了针对hgcA的新PCR引物组，hgcA是编码在广泛的系统发育范围内对Hg甲基化必不可少的类蛋氨酸蛋白的基因，并使用该引物组来研究hgcA序列在三个地点收集的土壤中硫酸盐和养分梯度的分布大沼泽地北部集中。获得的序列分布在不同的门中，包括变形杆菌，绿弯曲菌，硬毛菌和甲烷菌。但是，来自所有位点的hgcA克隆文库均以在Deltaproteobacteria的间质细菌顺序内聚集的序列（占总序列的49％至65％）为主导。从这些位点获得的代表活性的硫酸盐还原原核生物的dsrB mRNA序列也由滑膜细菌占主导（75％至89％）。实验室从硫酸盐浓度低的土壤中进行的温育还表明，汞甲基化活性主要由同食细菌的成员介导，其中甲烷菌，绿叶弯曲杆菌，铁还原性土杆菌和非硫酸盐类Firmicutes有一定贡献。网站。这表明分布在同食菌定义的进化枝内的原核生物是控制大沼泽地低硫酸盐地区汞甲基化的主要基团。在大沼泽地地区管理汞甲基化的任何策略都应考虑到净汞甲基化不仅限于硫酸盐还原作用。

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