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首页> 美国卫生研究院文献>Acta Veterinaria Scandinavica >Challenges for bovine viral diarrhoea virus antibody detection in bulk milk by antibody enzyme-linked immunosorbent assays due to changes in milk production levels
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Challenges for bovine viral diarrhoea virus antibody detection in bulk milk by antibody enzyme-linked immunosorbent assays due to changes in milk production levels

机译：牛奶产量水平变化对通过抗体酶联免疫吸附法检测散装牛奶中牛病毒性腹泻病毒抗体的挑战

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BackgroundBovine viral diarrhoea (BVD) is considered eradicated from Denmark. Currently, very few (if any) Danish cattle herds could be infected with BVD virus (BVDV). The Danish antibody blocking enzyme-linked immunosorbent assay (ELISA) has been successfully used during the Danish BVD eradication program, initiated in 1994. During the last decade, the cattle herd size has increased while the prevalence of BVDV has decreased. In this study, we investigated how these changes could affect the performance of the Danish blocking ELISA and of the SVANOVIR®BVDV-Ab indirect ELISA. The latter has successfully been used to eradicate BVD in Sweden.Data (2003–2010) on changes in median herd size and milk production levels, occurrence of viremic animals and bulk milk surveillance were analysed. Additionally, the Danish blocking ELISA and the SVANOVIR ELISA were compared analyzing milk and serum samples. The prevalence of antibody positive milking cows that could be detected by each test was estimated, by diluting positive individual milk samples and making artificial milk pools.
机译：背景牛病毒性腹泻（BVD）被认为已从丹麦根除。目前，很少（如果有的话）丹麦牛群会感染BVD病毒（BVDV）。丹麦抗体阻断酶联免疫吸附试验（ELISA）已成功用于1994年发起的丹麦BVD根除计划中。在过去十年中，牛群规模有所增加，而BVDV的患病率却有所下降。在这项研究中，我们研究了这些变化如何影响丹麦封闭ELISA和SVANOVIR ® BVDV-Ab间接ELISA的性能。后者已成功用于根除瑞典的BVD。分析了牛群中位数和产奶量变化，病毒血症动物的发生以及散装牛奶监测的数据（2003-2010年）。此外，比较了丹麦阻断ELISA和SVANOVIR ELISA在分析牛奶和血清样品中的作用。通过稀释阳性个体奶样品并建立人工奶池，估算了每次检测可检测到的抗体阳性奶牛的患病率。

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