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首页> 美国卫生研究院文献>Acta Neuropathologica Communications >Ultrastructural differences in pretangles between Alzheimer disease and corticobasal degeneration revealed by comparative light and electron microscopy
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Ultrastructural differences in pretangles between Alzheimer disease and corticobasal degeneration revealed by comparative light and electron microscopy

机译：通过比较光镜和电镜观察到阿尔茨海默氏病和皮质基底变性之间前缠结的超微结构差异

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Pretangles are defined under the light microscope as diffuse and granular tau immunoreactivity in neurons in tissue from patients with Alzheimer disease (AD) or corticobasal degeneration (CBD) and are considered to be a premature stage before neurofibrillary tangle formation. However, the ultrastructure of pretangles remains to be described. To clarify the similarities and differences between pretangles from patients with AD and CBD (AD-pretangles and CBD-pretangles, respectively), we examined cortical pretangles in tissue from patients with each of diseases. For direct light and electron microscopic (LM/EM) correlation of the pretangles, we used quantum dot nanocrystals (QDs) with dual fluorescent and electron-dense properties. We first identified tau-labeled pretangles on fluorescence LM and subsequently examined the same neurons on EM. Energy dispersive X-ray spectrometry (EDX) color mapping identified selenium (Se) and cadmium (Cd) as elementary components of QDs and highlighted each QD particle clearly against gray-scale EM images. With these methods, we were successful for the first time in demonstrating accurately that LM-defined pretangles are tau-positive straight filaments sparsely distributed throughout neuronal cytoplasm and neurites in both AD and CBD at the EM level. Notably, AD-pretangles showed a strong tendency to form fibrillary tangles even at an early stage, whereas pretangles or Pick-like inclusions in tissue from patients with CBD did not even at an advanced stage. In conclusion, AD-pretangles and CBD-pretangles showed essential differences at the EM level.Electronic supplementary materialThe online version of this article (doi:10.1186/s40478-014-0161-3) contains supplementary material, which is available to authorized users.
机译：在光学显微镜下，缠结被定义为阿尔茨海默病（AD）或皮质基底突变性（CBD）患者组织中神经元的弥漫性和颗粒状tau免疫反应性，被认为是神经原纤维缠结形成之前的早熟阶段。但是，前缠结的超微结构仍有待描述。为了阐明AD和CBD患者的前缠结之间的异同（分别为AD前缠结和CBD前缠结），我们检查了每种疾病患者的组织中的皮质前缠结。对于预缠结的直接光和电子显微镜（LM / EM）相关性，我们使用了具有双重荧光和电子致密特性的量子点纳米晶体（QD）。我们首先在荧光LM上鉴定了tau标记的缠结，然后在EM上检查了相同的神经元。能量色散X射线光谱（EDX）色彩映射将硒（Se）和镉（Cd）识别为QD的基本成分，并针对灰度EM图像清晰地突出显示每个QD粒子。通过这些方法，我们首次成功地准确证明了LM定义的缠结是tau阳性的直丝，稀疏分布在整个EM水平的AD和CBD的神经元细胞质和神经突中。值得注意的是，AD前缠结即使在早期也显示出强烈的纤维缠结趋势，而CBD患者组织中的前缠结或Pick-like夹杂物甚至在晚期都没有。总之，AD矩形和CBD矩形在EM级别上显示出本质上的区别。电子补充材料本文的在线版本（doi：10.1186 / s40478-014-0161-3）包含补充材料，授权用户可以使用。

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