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首页> 美国卫生研究院文献>International Journal of Clinical and Experimental Medicine >Effects of Shenqi Neijin powder on activation and apoptosis of hepatic stellate cells in rats with hepatic fibrosis
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Effects of Shenqi Neijin powder on activation and apoptosis of hepatic stellate cells in rats with hepatic fibrosis

机译：参芪内劲散对肝纤维化大鼠肝星状细胞活化和凋亡的影响。

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摘要

Traditional herbal medicine is usually administrated according to experiences and practices. We aimed to investigate the anti-fibrotic effects of Shenqi Neijin powder (SQNJP) in hepatic fibrosis rats induced by carbon tetrachloride (CCl4). A total of 32 rats were divided into control group, model group, and SQNJP group. The hydroxyproline content was assayed. Histological features of liver tissues were determined with different staining methods. Western blotting analysis and immuno-fluorescence staining were performed to determine the expression of alpha-smooth muscle actin (α-SMA) and the activation of hepatic stellate cells (HSCs). Serial sections were stained with α-SMA immuno-fluorescence staining and the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling method (TUNEL) in turn to detect the apoptosis of HSCs. Fatty degeneration, deposition of collagen, and interval of fibers were noticed in rats induced by CCl4. After administration of SQNJP, remarkable decrease of fatty degeneration, deposition of collagen, and hydroxyproline content were noticed. Compared with the model group, significant decrease of α-SMA protein was noticed after administration of SQNJP, and remarkable apoptosis of HSCs was noticed after treating with SQNJP. SQNJP showed anti-fibrotic effects through inhibiting HSCs activation and inducing apoptosis of HSCs.
机译：传统草药通常根据经验和做法进行管理。我们旨在研究参芪内金粉（SQNJP）对四氯化碳（CCl4）诱导的肝纤维化大鼠的抗纤维化作用。将32只大鼠分为对照组，模型组和SQNJP组。测定羟脯氨酸含量。用不同的染色方法确定肝组织的组织学特征。进行了蛋白质印迹分析和免疫荧光染色，以确定α-平滑肌肌动蛋白（α-SMA）的表达和肝星状细胞（HSC）的激活。连续切片用α-SMA免疫荧光染色和末端脱氧核苷酸转移酶介导的dUTP-生物素缺口末端标记法（TUNEL）进行染色，以检测HSC的凋亡。在CCl4诱导的大鼠中发现了脂肪变性，胶原蛋白沉积和纤维间隔。服用SQNJP后，发现脂肪变性，胶原蛋白沉积和羟脯氨酸含量显着降低。与模型组相比，SQNJP给药后α-SMA蛋白显着下降，SQNJP治疗后HSCs明显凋亡。 SQNJP通过抑制HSC的活化和诱导HSC的凋亡而显示出抗纤维化作用。

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