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Influence of different concentrations of uric acid on oxidative stress in steatosis hepatocytes

机译：不同浓度尿酸对脂肪变性肝细胞氧化应激的影响

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摘要

The development of nonalcoholic fatty liver disease (NAFLD) is caused by the steatosis of hepatocytes, which induces oxidative stress (OS). Thus, OS has an important role in the development of NAFLD. In the present study, the L-02 hepatocyte cell line was used to develop a steatosis cell model. The best model was determined using an MTT assay and the triglyceride levels. Model cells were treated with high concentrations of uric acid (UA; 0, 5, 10, 20 and 30 mg/dl) for 24, 48, 72 and 96 h. Indicators of oxidation were then measured, which included total superoxide dismutase (SOD), malonaldehyde (MDA) and reduced glutathione (GSH), and the transcriptional and translational levels of SOD1 and γ-glutamate-cysteine ligase (γ-GCLC) were also determined. In addition, the intracellular levels of aspartate aminotransferase and alanine aminotransferase (ALT) were detected. The activity of SOD1 decreased over time and the result was supported by the results of western blotting. The transcriptional levels of SOD1 in model cells was significantly higher than untreated cells at 48 h. With the decreased levels of SOD1 and GSH, MDA increased in a time-dependent manner. The content of GSH decreased with time as well, which was also reflected in the results of western blotting. The transcriptional levels of γ-GCLC in all UA-treated groups were lower when compared with those observed in the model group. The activity of ALT tended to increase, depending on the duration of treatment. Treatment with 5 and 10 mg/dl UA had an antioxidative effect on the model cells, and 30 mg/dl UA treatment for 48 h increased OS in the cells.
机译：非酒精性脂肪肝疾病（NAFLD）的发展是由肝细胞脂肪变性引起的，该脂肪变性会诱发氧化应激（OS）。因此，操作系统在NAFLD的开发中具有重要作用。在本研究中，L-02肝细胞细胞系用于建立脂肪变性细胞模型。使用MTT测定法和甘油三酸酯水平确定了最佳模型。用高浓度的尿酸（UA； 0、5、10、20和30 mg / dl）处理模型细胞24、48、72和96 h。然后测量氧化指示剂，包括总超氧化物歧化酶（SOD），丙二醛（MDA）和还原型谷胱甘肽（GSH），还确定了SOD1和γ-谷氨酸-半胱氨酸连接酶（γ-GCLC）的转录和翻译水平。 。另外，检测了细胞内天冬氨酸转氨酶和丙氨酸转氨酶（ALT）的水平。 SOD1的活性随着时间的推移而下降，这一结果得到了蛋白质印迹法的支持。在48小时时，模型细胞中SOD1的转录水平显着高于未处理的细胞。随着SOD1和GSH含量的降低，MDA呈时间依赖性增加。谷胱甘肽的含量也随着时间而减少，这也反映在蛋白质印迹的结果中。与模型组相比，所有UA处理组的γ-GCLC转录水平均较低。 ALT的活性倾向于增加，这取决于治疗的持续时间。 5和10 mg / dl UA处理对模型细胞具有抗氧化作用，而30 mg / dl UA处理48 h可提高细胞的OS。

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