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首页> 美国卫生研究院文献>Microbiology >Use of a stainless steel washer platform to study Acinetobacter baumannii adhesion and biofilm formation on abiotic surfaces
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Use of a stainless steel washer platform to study Acinetobacter baumannii adhesion and biofilm formation on abiotic surfaces

机译：使用不锈钢清洗机平台研究鲍曼不动杆菌在非生物表面上的粘附和生物膜形成

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Acinetobacter baumannii is a frequent cause of hospital-acquired pneumonia, and has recently increased in incidence as the causative agent of severe disease in troops wounded in Afghanistan and Iraq. Clinical approaches are limited since A. baumannii strains isolated from patients are extremely resistant to current antimicrobials. A. baumannii can survive desiccation and during outbreaks has been recovered from various sites in the patients’ environment. To better understand its prevalence in hospital settings, we used a stainless steel washer (SSW) platform to investigate A. baumannii biofilm formation on abiotic surfaces. Scanning electron microscopy demonstrated that A. baumannii forms strong biofilms on stainless steel surfaces. This platform was combined with a colorimetric 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazolium hydroxide (XTT) reduction assay to observe the metabolic activity of bacterial cells, and to facilitate the manipulation and comparison of multiple A. baumannii clinical strains. A strong correlation between XTT and c.f.u. assays was demonstrated. To complement the cell viability assays, A. baumannii biofilm mass was measured by crystal violet staining. Furthermore, the effect of commonly used disinfectants and environmental stressors on A. baumannii biofilms and planktonic cells was compared and characterized. Biofilms on SSWs were significantly more resistant than their planktonic counterparts, providing additional evidence that may allow us to understand the high prevalence of this microbe in hospital settings. Our results validate that SSWs are a simple, versatile and innovative method to study A. baumannii biofilms in vitro.
机译：鲍曼不动杆菌是医院获得性肺炎的常见病因，最近作为阿富汗和伊拉克受伤人员中严重疾病的病因，其发病率有所增加。由于从患者中分离出的鲍曼不动杆菌菌株对目前的抗菌剂极具抵抗力，因此临床方法受到限制。鲍曼不动杆菌可以在干燥中幸存下来，并且在暴发期间已从患者环境中的各个位置恢复过来。为了更好地了解其在医院环境中的流行程度，我们使用了不锈钢洗衣机（SSW）平台来研究鲍曼不动杆菌在非生物表面上的生物膜形成。扫描电子显微镜显示鲍曼不动杆菌在不锈钢表面上形成坚固的生物膜。该平台与比色的2,3-双（2-甲氧基-4-硝基-5-磺基苯基）-5-[（苯基氨基）羰基] -2H-氢氧化四唑（XTT）还原试验相结合，观察其代谢活性。细菌细胞，并有助于多种鲍曼不动杆菌临床菌株的操作和比较。 XTT与c.f.u之间有很强的相关性。实验证明。为了补充细胞活力测定，通过结晶紫染色测量鲍曼不动杆菌生物膜质量。此外，比较和表征了常用消毒剂和环境胁迫对鲍曼不动杆菌生物膜和浮游细胞的影响。 SSWs上的生物膜比浮游生物具有更大的抵抗力，这提供了更多的证据，使我们可以了解这种微生物在医院中的高流行率。我们的结果证实，SSWs是一种简单，通用和创新的方法，可以在体外研究鲍曼不动杆菌生物膜。

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