• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Scientific Reports >Detection of Cronobacter sakazakii in powdered infant formula using an immunoliposome-based immunomagnetic concentration and separation assay
【2h】

Detection of Cronobacter sakazakii in powdered infant formula using an immunoliposome-based immunomagnetic concentration and separation assay

机译：基于免疫脂质体的免疫磁浓度和分离法检测婴儿配方奶粉中的阪崎肠杆菌

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

This study aimed to optimize the applicability of an immunoliposome-based immunomagnetic concentration and separation assay to facilitate rapid detection of Cronobacter sakazakii in powdered infant formula (PIF). To determine the detection limit, specificity, and pre-enrichment incubation time (0, 4, 6, and 8 h), assay tests were performed with different cell numbers of C. sakazakii (2 × 100 and 2 × 101 CFU/ml) inoculated in 10 g of PIF. The assay was able to detect as few as 2 cells of C. sakazakii/10 g of PIF sample after 6 h of pre-enrichment incubation with an assay time of 2 h 30 min. The assay was assessed for cross-reactivity with other bacterial strains and exhibited strong specificity to C. sakazakii. Moreover, the assay method was applied to the detection of C. sakazakii in PIF without pre-enrichment steps, and the results were compared with INC-ELISA and RT-PCR. The developed method was able to detect C. sakazakii in spiked PIF without pre-enrichment, whereas INC-ELISA failed to detect C. sakazakii. In addition, when compared with the results obtained with RT-PCR, our developed assay required lesser detection time. The developed assay was also not susceptible to any effect of the food matrix or background contaminant microflora.
机译：这项研究旨在优化基于免疫脂质体的免疫磁浓度和分离测定法的适用性，以促进快速检测婴儿配方奶粉（PIF）中的阪崎肠杆菌。为了确定检出限，特异性和富集前孵育时间（0、4、6和8 h），对阪崎肠杆菌的不同细胞数（2×10 0 ）进行测定测试在10μgPIF中接种2×10 1 CFU / ml）。在预富集孵育6µh后，检测时间为2µh 30µmin，该检测法能够检测出仅2个阪崎肠杆菌/ 10µg PIF样品细胞。评估了该测定法与其他细菌菌株的交叉反应性，并显示出对阪崎肠杆菌的强特异性。此外，该测定方法适用于无需预富集步骤的PIF中阪崎肠杆菌的检测，并将结果与​​INC-ELISA和RT-PCR进行了比较。所开发的方法能够在不预先富集的情况下检测加标PIF中的阪崎肠杆菌，而INC-ELISA无法检测到阪崎肠杆菌。此外，与RT-PCR获得的结果相比，我们开发的测定方法需要更短的检测时间。发达的检测方法也不易受到食物基质或背景污染物菌群的任何影响。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号