• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Scientific Reports >EUF1 – a newly identified gene involved in erythritol utilization in Yarrowia lipolytica
【2h】

EUF1 – a newly identified gene involved in erythritol utilization in Yarrowia lipolytica

机译：EUF1 –一个新发现的与解脂耶氏酵母中赤藓糖醇利用有关的基因

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The gene YALI0F01562g was identified as an important factor involved in erythritol catabolism of the unconventional yeast Yarrowia lipolytica. Its putative role was identified for the first time by comparative analysis of four Y. lipolytica strains: A-101.1.31, Wratislavia K1, MK1 and AMM. The presence of a mutation that seriously damaged the gene corresponded to inability of the strain Wratislavia K1 to utilize erythritol. RT-PCR analysis of the strain MK1 demonstrated a significant increase in YALI0F01562g expression during growth on erythritol. Further studies involving deletion and overexpression of the selected gene showed that it is indeed essential for efficient erythritol assimilation. The deletion strain Y. lipolytica AMM∆euf1 was almost unable to grow on erythritol as the sole carbon source. When the strain was applied in the process of erythritol production from glycerol, the amount of erythritol remained constant after reaching the maximal concentration. Analysis of the YALI0F01562g gene sequence revealed the presence of domains characteristic for transcription factors. Therefore we suggest naming the studied gene Erythritol Utilization Factor – EUF1.
机译：YALI0F01562g基因被确定为非常规酵母解脂耶氏酵母的赤藓糖醇分解代谢的重要因素。通过对四种解脂耶氏酵母菌株：A-101.1.31，Wratislavia K1，MK1和AMM的比较分析，首次确定了其推定作用。严重破坏基因的突变的存在对应于菌株Wratislavia K1无法利用赤藓醇。菌株MK1的RT-PCR分析表明，赤藓糖醇生长期间YALI0F01562g表达显着增加。涉及缺失和过表达所选基因的进一步研究表明，它对于有效的赤藓糖醇同化确实必不可少。缺失菌株解脂耶氏酵母AMM∆euf1几乎不能在赤藓糖醇作为唯一碳源上生长。当该菌株用于由甘油生产赤藓糖醇的过程中时，赤藓糖醇的量在达到最大浓度后保持恒定。对YALI0F01562g基因序列的分析揭示了转录因子特征域的存在。因此，我们建议命名研究的基因赤藓糖醇利用因子– EUF1。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号