• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Scientific Reports >De novo assembly and characterization of root transcriptome in two distinct morphotypes of vetiver Chrysopogon zizaniodes (L.) Roberty
【2h】

De novo assembly and characterization of root transcriptome in two distinct morphotypes of vetiver Chrysopogon zizaniodes (L.) Roberty

机译：香根草（Chrysopogon zizaniodes（L.）Roberty）的两种不同形态类型的根转录组的从头组装和表征

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Vetiver, a perennial C4 grass, has long been known for its multifarious uses in perfumery, medicine and environmental protection. Two distinct vetiver morphotypes have been identified in India, i.e., A. North Indian type characterized by thick and smooth fast growing roots that produce superior quality of laevorotatory oil; and B. South Indian type with more number of thin and hairy roots that produce inferior quality of dextrorotatory oil. The two morphotypes were targeted for transcriptome analysis to understand the contribution of genetic background on oil quality and root morphology. Sample A showed enhanced activity of flavonoid and terpenoid biosynthesis related genes, i.e. ERF, MYB, bHLH, bZIP and WRKY. Interestingly, expression analysis revealed that the genes involved in sesquiterpene biosynthesis pathway were up regulated in Sample A. Moreover, some of the genes involved in mevalonate pathway of sesquiterpene biosynthesis were unique to Sample A. Our results also demonstrated several transcripts involved in root development and hormonal regulation being up regulated in Sample A. To validate gene expression results of RNA-seq data, 20 transcripts were validated by qRT-PCR experiment. The present study provided an important start point for further discovery of genes related to root oil quality in different ecotypes of vetiver.
机译：香根草是多年生的C4草，长期以来以其在香水，药品和环境保护中的广泛用途而闻名。印度已鉴定出两种不同的香根草形态型，即A.北印度型，其特征是浓密而光滑的快速生长的根，产生优质的立叶油。 B.南印度型，根系稀疏，毛茸茸，产生的右旋油质量较差。将这两种形态型用于转录组分析，以了解遗传背景对油品质和根形态的贡献。样品A显示出类黄酮和类萜生物合成相关基因，即ERF，MYB，bHLH，bZIP和WRKY的活性增强。有趣的是，表达分析表明，倍半萜生物合成途径中涉及的基因在样品A中被上调。此外，倍半萜生物合成中甲羟戊酸途径中的一些基因是样品A特有的。样品A中的激素调节被上调。为了验证RNA-seq数据的基因表达结果，通过qRT-PCR实验验证了20个转录本。本研究为进一步发现香根草不同生态类型中与根油品质有关的基因提供了重要的起点。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号